Báo cáo khoa học: Mutations in the C-terminal domain of ALSV (Avian Leukemia and Sarcoma Viruses) integrase alter the concerted DNA integration process in vitro
43
lượt xem 4
download
lượt xem 4
download
Download
Vui lòng tải xuống để xem tài liệu đầy đủ
Integrase (IN) is the retroviral enzyme responsible for the integration of the DNA copy of the retroviral genome into the host cellDNA. TheC-terminal domainof INis involved in DNA binding and enzyme multimerization. We previ-ously performed single amino acid substitutions in the C-terminal domain of the avian leukemia and sarcoma vir-uses (ALSV) IN [Moreauet al. (2002). Arch. Virol.147, 1761–1778]. Here, we modelled these IN mutants and ana-lysed their ability tomediate concertedDNA integration (in anin vitroassay) as well as to formdimers (by size exclusion chromatography and protein–protein cross-linking). ...
Chủ đề:
Bình luận(0) Đăng nhập để gửi bình luận!
CÓ THỂ BẠN MUỐN DOWNLOAD