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Báo cáo khóa học: Mutations in the hydrophobic core and in the protein–RNA interface affect the packing and stability of icosahedral viruses

Chia sẻ: Nguyen Thang | Ngày: | Loại File: PDF | Số trang:11

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The information required for successful assembly of an icosahedral virus is encoded in the native conformation of the capsidproteinand in its interactionwith the nucleic acid. Here we investigated how the packing and stability of virus capsids are sensitive to single amino acid substitutions in the coat protein. Tryptophan fluorescence, bis-8-anilinonaph-thalene-1-sulfonate fluorescence, CD and light scattering were employed to measure urea- and pressure-induced effects on MS2 bacteriophage and temperature sensitive mutants. ...

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Nội dung Text: Báo cáo khóa học: Mutations in the hydrophobic core and in the protein–RNA interface affect the packing and stability of icosahedral viruses

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