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Báo cáo khoa học: Possible involvement of an FKBP family member protein from a psychrotrophic bacterium Shewanella sp. SIB1 in cold-adaptation

Chia sẻ: Nguyen Thang | Ngày: | Loại File: PDF | Số trang:10

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Apsychrotrophic bacteriumShewanellasp. strain SIB1 was grown at 4 and 20C, and total soluble proteins extracted from the cells were analyzed by two-dimensional poly-acrylamide gel electrophoresis. Comparison ofthese pat-terns showed that the cellular content ofa protein with a molecular mass of28 kDa and an isoelectric point of four greatly increased at 4 C compared to that at 20C. Determination ofthe N-terminal amino acid sequence, fol-lowed by the cloning and sequencing ofthe gene encoding this protein, revealed that this protein is a member ofthe FKBP family of proteins with an amino acid sequence identity of56% to Escherichia coliFKBP22. ...

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Nội dung Text: Báo cáo khoa học: Possible involvement of an FKBP family member protein from a psychrotrophic bacterium Shewanella sp. SIB1 in cold-adaptation

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