Báo cáo khoa học: Properties of the recombinant glucose⁄galactose dehydrogenase from the extreme thermoacidophile, Picrophilus torridus
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In Picrophilus torridus, a euryarchaeon that grows optimally at 60 C and pH 0.7 and thus represents the most acidophilic thermophile known, glu-cose oxidation is the first proposed step of glucose catabolism via a non-phosphorylated variant of the Entner–Doudoroff pathway, as deduced from the recently completed genome sequence of this organism. The P. torridusgene for a glucose dehydrogenase was cloned and expressed in Escherichia coli, and the recombinant enzyme, GdhA, was purified and characterized....
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