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Báo cáo khoa học: Proteolytic processing of porcine deoxyribonuclease II occurs in lysosomes but is not required for enzyme activation
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DNase II purified from porcine spleen (pDNase II) comprisesa1, a2 and bsubunits. The three subunits are encoded by one cDNA, in the sequence a1 , b, and a2, and the peptides linking these subunits are presumably cleaved out post-translationally. To understand the relevance of post-trans-lational cleavage to pDNase II, recombinant pDNase II (rpDNase II) was produced in human 293T cells by transfection with an expression plasmid containing pDNase II cDNA (pcDNaseII).
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