Báo cáo khoa học: Relationship between the structure of guanidines and N-hydroxyguanidines, their binding to inducible nitric oxide synthase (iNOS) and their iNOS-catalysed oxidation to NO
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The binding of several alkyl- and aryl-guanidines andN-hydroxyguanidines to the oxygenase domain of inducible NO-synthase (iNOSoxy) was studied by UV⁄Vis difference spectroscopy. In a very general manner, monosubsti-tuted guanidines exhibited affinities for iNOSoxy that were very close to those of the corresponding N-hydroxyguanidines. The highest affinities were observed for the natural substrates,l-arginine and N x -hydroxy-l-arginine (Kd at thelmlevel).
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