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Báo cáo khoa học: S -Stereoselective piperazine-2-tert-butylcarboxamide hydrolase from Pseudomonas azotoformans IAM 1603 is a novel L-amino acid amidase

Chia sẻ: Nguyen Thang | Ngày: | Loại File: PDF | Số trang:11

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An amidase acting on (R,S)-piperazine-2-tert-butylcarbox-amide was purified fromPseudomonas azotoformansIAM 1603 and characterized. The enzyme actedS-stereoselec-tively on (R,S)-piperazine-2-tert-butylcarboxamide to yield (S)-piperazine-2-carboxylic acid. N-terminal and internal amino acid sequences of the enzyme were determined. ThegeneencodingtheS-stereoselective piperazine-2-tert-butylcarboxamide amidase was cloned from the chromo-somal DNAof the strain and sequenced.

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Nội dung Text: Báo cáo khoa học: S -Stereoselective piperazine-2-tert-butylcarboxamide hydrolase from Pseudomonas azotoformans IAM 1603 is a novel L-amino acid amidase

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