Báo cáo khoa học: Signal peptide hydrophobicity is critical for early stages in protein export by Bacillus subtilis
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Signal peptides that direct protein export inBacillus subtilisare overall more hydrophobic than signal peptides inEscherichia coli. To study the importance of signal peptide hydrophobicity for protein export in both organisms, the a-amylase AmyQ was provided with leucine-rich (high hydrophobicity) or alanine-rich (low hydrophobicity) signal peptides. AmyQ export was most efficiently directed by the authentic signal peptide, both in E. coliand B. subtilis.
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