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Báo cáo khoa học: Substrate recognition and ®delity of strand joining by an archaeal DNA ligase
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We have previously identi®ed a DNA ligase (LigTk)froma hyperthermophilic archaeon,Thermococcus kodakaraensis KOD1. The enzyme is the only characterized ATP-depend-ent DNA ligase from a hyperthermophile, and allows the analysis of enzymatic DNA ligation reactions at tempera-tures above themeltingpoint of the substrates.Herewe have focused on the interactions of LigTkwith various DNA substrates, and its speci®cities toward metal cations.
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