Báo cáo khoa học: Temperature and phosphate effects on allosteric phenomena of phosphofructokinase from a hibernating ground squirrel (Spermophilus lateralis)
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Temperature effects on the kinetic properties of phosphofructokinase (PFK) purified from skeletal muscle of the golden-mantled ground squirrel, Spermophilus lateralis, were examined at 37 C and 5C, values character-istic of body temperatures in euthermia vs. hibernation. The enzyme showed reduced sensitivity to all activators at 5C, theKa values for AMP, ADP, NH4 + and F2,6P2 were 3–11-fold higher at 5C than at 37C. Inhibition by citrate was not affected whereas phosphoenolpyruvate, ATP and urea became more potent inhibitors at low temperature. ...
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