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Báo cáo khoa học: The duality of LysU, a catalyst for both Ap4A and Ap3A formation
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Heat shock inducible lysyl-tRNA synthetase ofEscherichia coli(LysU) is known to be a highly efficient diadenosine 5¢,5¢¢¢-P 1 ,P 4 -tetraphosphate (Ap4A) synthase. However, we use an ion-exchange HPLC technique to demonstrate that active LysU mixtures actually have a dual catalytic activ-ity, initially producing Ap4 A from ATP, before converting that tetraphos-phate to a triphosphate.
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