Báo cáo khoa học: Thermoanaerobacterium thermosulfurigenes cyclodextrin glycosyltransferase Mechanism and kinetics of inhibition by acarbose and cyclodextrins

Cyclodextrin glycosyltransferase (CGTase) uses ana-retain-ingdoubledisplacementmechanismtocatalyze threedistinct transglycosylation reactions. To investigate these reactions as catalyzed by the CGTase fromThermoanaerobacterium thermosulfurigenesthe enzyme was overproduced (8 mgÆL )1 culture) usingBacillus subtilisas a host. Detailed analysis revealed that the three reactions proceed via different kinetic mechanisms.