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Báo cáo khoa học: X-ray crystallography, CD and kinetic studies revealed the essence of the abnormal behaviors of the cytochrome b5 Phe35fiTyr mutant

Chia sẻ: Nguyen Thang | Ngày: | Loại File: PDF | Số trang:10

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Conservedphenylalanine 35 is one of the hydrophobic patch residues on the surface of cytochromeb5 (cytb5). This patch is partially exposed on the surface of cyt b5while its buried face is in direct van der Waals’contact with heme b. Resi-dues Phe35 and Phe/Tyr74 also form an aromatic channel with His39, which is one of the axial ligands of heme b. By site-directedmutagenesiswehaveproduced threemutantsof cytb5:Phe35fiTyr, Phe35fiLeu, and Phe35fiHis.

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Nội dung Text: Báo cáo khoa học: X-ray crystallography, CD and kinetic studies revealed the essence of the abnormal behaviors of the cytochrome b5 Phe35fiTyr mutant

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