Báo cáo Y học: Assignment of molecular properties of a superactive coagulation factor VIIa variant to individual amino acid changes

Eur. J. Biochem. 269, 5950–5955 (2002) Ó FEBS 2002 doi:10.1046/j.1432-1033.2002.03323.x The most active factor VIIa (FVIIa) variants identified to date carry concurrent substitutions at positions 158, 296and 298 with the intention of generating a thrombin-mimicking motif, optionally combined with additional replacements within the protease domain [Persson, E., Kjalke, M. & Olsen, O. H. (2001)Proc. Natl Acad. Sci. USA98, 13583– 13588]. Here we have characterized variants of FVIIa mutatedat one or twoof these positions toassess the relative importance of the individual replacements. Assignment of molecular properties of a superactive coagulation factor VIIa variant to individual amino acid changes Egon Persson1 and Ole H. Olsen2 1 Haemostasis Biology and 2Medicinal Chemistry Research IV, Novo Nordisk A/S,...