intTypePromotion=1
zunia.vn Tuyển sinh 2024 dành cho Gen-Z zunia.vn zunia.vn
ADSENSE

Báo cáo Y học: Cloning, expression and characterization of a gene encoding nitroalkane-oxidizing enzyme from Streptomyces ansochromogenes

Chia sẻ: Nguyen Thang | Ngày: | Loại File: PDF | Số trang:6

45
lượt xem
2
download
 
  Download Vui lòng tải xuống để xem tài liệu đầy đủ

A nitroalkane-oxidizing enzyme gene (naoA) was cloned from a genomic DNA library ofStreptomyces ansochromo-genes7100. The deduced protein (NaoA) of this gene con-tains 363 amino acids and has high similarity to several nitroalkane-oxidizing enzymes from various micro-organ-isms.ThenaoAgenewas subcloned intoanexpressionvector pET23b and overexpressed inEscherichia coliBL21(DE3). The protein was then purified, and its characteristics were studied. Experimental results showed that NaoA can con-vert 1-nitropropane, 2-nitropropane and nitroethane into the corresponding carbonyl compounds....

Chủ đề:
Lưu

Nội dung Text: Báo cáo Y học: Cloning, expression and characterization of a gene encoding nitroalkane-oxidizing enzyme from Streptomyces ansochromogenes

ADSENSE

CÓ THỂ BẠN MUỐN DOWNLOAD

 

Đồng bộ tài khoản
2=>2