Báo cáo Y học: Intracellular localization and transcriptional regulation of tumor necrosis factor (TNF) receptor-associated factor 4 (TRAF4)

To gain insight in the subcellular localization of tumor necrosis factor receptor-associated factor (TRAF4) we analyzed GFP chimeras of full-length TRAF4 and various deletionmutants derived thereof.While TRAF4–GFP (T4– GFP) was clearlylocalized in the cytoplasm, the N-terminal deletion mutant, T4(259–470), comprising the TRAF domain of the molecule, and a C-terminal deletion mutant consisting mainlyof the RING and zinc finger domains of TRAF4 were both localized predominantlyto the nucleus....