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Báo cáo Y học: Role of electrostatics in the interaction between plastocyanin and photosystem I of the cyanobacterium Phormidium laminosum
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The interactions between photosystem I and five charge mutants of plastocyanin from the cyanobacteriumPhormi-dium laminosumwere investigatedin vitro. The dependence of the overall rate constant of reaction,k2, on ionic strength was investigated using laser flash photolysis. The rate con-stant of the wild-type reaction increased with ionic strength, indicating repulsion between the reaction partners. Remov-inganegativechargeonplastocyanin(D44A) acceleratedthe reactionandmade it independentof ionic strength; removing a positive charge adjacent to D44 (K53A) had little effect....
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