Detection of mycobacterium tuberculosis from clinical samples using different real-time PCR system

During the study period (July to December 2016) a total of 188 samples (sputum and blood from each person) have been tested in the National Reference Laboratory (NRL) at the Institute of Chest and Respiratory Diseases / Baghdad. These cases are considered as suspected pulmonary tuberculosis. This study for these 188 suspected specimens declare that, 103 (54.78%) cases were positive using direct assay (AFB smear microscopy) and 85 (45.21%) were considered to be negative cases by smear microscopy.