Development of an indirect enzyme-linked immunosorbent assay using recombinant microneme 10 (MIC10) protein of toxoplasma gondii for diagnostic evaluation in murine model infected with tachyzoites

The aim of present study was to express the microneme 10 (MIC10) antigenic protein which is a circulating antigen following invasion by tachyzoites in hosts in the prokaryotic expression system and evaluated as a potential diagnostic marker by indirect ELISA using the infected and non infected sera samples from Swiss albino mice. Optimum working dilutions of the antigen, sera and the conjugate were determined by checker board titrations and the cut off value was generated as mean plus standard deviation of optical densities of negative sera by ELISA.