Expression profiling of resistance gene analogs from French bean (Phaseolus vulgaris L.) for the manifestation of moong bean yellow mosaic virus

The popular and susceptible french bean genotype Arka Anoop was used for expression profiling of RGAs for the manifestation of MYMV. Leaf tissue was collected from both disease free and artificially disease challenged plants at 7 th, 15th and 30th DAI and syntesized cDNA. The expression level of selected 10 RGA genes of french bean was measured in both disease free and artificially inoculated leaf tissues separately at 7 th, 15th and 30th DAI. 10 RGA genes were tested under MYMV infested condition in french bean. All 10 selected RGA genes were expressed in leaf tissues at different days after inoculation with MYMV. COHFBRGA-2, 6, 8 and 10 genes of french bean were upregulated in leaf of susceptible genotypes at disease inoculation condition at all the intervals. COHFBRGA-3, 4, 5 and 7 was down- regulated among all the intervals of disease development compared to control. Whereas, COHFBRGA-9 expressed only at 30 DAI.