Expression profiling

Starch metabolism is known to be an important pathway in the growth and development of plants. This study was conducted to investigate the genome-wide identification and structural analysis of genes encoding uridine diphosphate glucose pyrophosphorylase (UGPase), a key enzyme in starch synthesis in cassava, and to analyze the expression profiles of these genes based on publicly available RNA-seq data.

6p vibecca 01-10-2024 0 0   Download

It is a basic task in high-throughput gene expression profiling studies to identify differentially expressed genes (DEGs) between two phenotypes. RankComp, an algorithm, could analyze the highly stable withinsample relative expression orderings (REOs) of gene pairs in a particular type of human normal tissue that are widely reversed in the cancer condition, thereby detecting DEGs for individual disease samples measured by a particular platform.

11p vielonmusk 21-01-2022 14 1   Download

Intrahepatic cholangiocarcinoma (ICC) is a type of malignant tumor ranking the second in the incidence of primary liver cancer following hepatocellular carcinoma. Both the morbidity and mortality have been increasing in recent years. Small duct type of ICC has potential therapeutic targets. But overall, the prognosis of patients with ICC is usually very poor.

12p vielonmusk 21-01-2022 17 1   Download

Glioblastoma (GBM) can be divided into subtypes according to their genomic features, includ‑ ing Proneural (PN), Neural (NE), Classical (CL) and Mesenchymal (ME). However, it is a difficult task to unify various genomic expression profiles which were standardized with various procedures from different studies and to manually classify a given GBM sample into a subtype.

13p vielonmusk 21-01-2022 16 1   Download

Hepatocellular carcinoma (HCC) is one of the deadliest cancers. Research on HCC so far primarily focused on genes and provided limited information on genomic repeats, which constitute more than half of the human genome and contribute to genomic stability. In line with this, repeat dysregulation was significantly shown to be pathological in various cancers and other diseases. In this study, we aimed to determine the full repeat expression profile of HCC for the first time.

14p thiencuuchu 27-11-2021 21 3   Download

Molecular knowledge regarding the primary esophageal achalasia is essential for the early diagnosis and treatment of this neurodegenerative motility disorder. Therefore, there is a need to find the main microRNAs (miRNAs) contributing to the mechanisms of achalasia. This study was conducted to determine some patterns of deregulated miRNAs in achalasia. This case-control study was performed on 52 patients with achalasia and 50 nonachalasia controls. The miRNA expression profiling was conducted on the esophageal tissue samples using the next-generation sequencing (NGS).

13p thiencuuchu 27-11-2021 9 2   Download

Stevia rebaudiana is a medicinal plant recommended to diabetic or obese patients as an alternative sweetener owing to its low-calorie property. Previous studies have found that the stevioside level is highest at the time of flower bud formation and lowest at the time of preceding and following flower bud formation. Hence, this study aims to identify the genes involved in the flowering of local S.

10p thiencuuchu 27-11-2021 12 0   Download

Understanding of the functions of microRNAs in breast cancer and breast cancer stem cells have been a hope for the development of new molecular targeted therapies. Here, it is aimed to investigate the differences in the expression levels of let-7a, miR10b, miR-21, miR-125b, miR-145, miR-155, miR-200c, miR-221, miR-222 and miR-335, which associated with gene and proteins in MCF-7 (parental) and MCF-7s (Mammosphere/stem cell-enriched population/CD44+/CD24-cells) cells treated with paclitaxel. MCF7s were obtained from parental MCF-7 cells.

12p thiencuuchu 27-11-2021 16 2   Download

Calmodulin (CaM) is an important calcium sensor protein that transduces Ca2+ signals in plant stress signaling pathways. A previous study has revealed that transgenic rice over-expressing the calmodulin gene OsCam1–1 (LOC_Os03g20370) is more tolerant to salt stress than wild type.

23p vishikamaru2711 29-04-2020 13 1   Download

The auxin response factor (ARF) gene family is involved in plant development and hormone regulation. Although the ARF gene family has been studied in some plant species, its structural features, molecular evolution, and expression profiling in Brachypodium distachyon L. are still not clear.

15p vishikamaru2711 29-04-2020 31 2   Download

MicroRNAs (miRNAs) are small noncoding RNAs of about 19–25 nt that regulate gene expression posttranscriptionally under various cellular conditions, including apoptosis. The miRNAs involved in modulation of apoptotic events in T cells are partially known.

10p nutifooddau 27-01-2019 23 2   Download

Genomicfattyacid elongation 1 (FAE1) clones from high erucic acid (HEA)Brassica napus, Brassica rapaandBras-sicaoleracea, and lowerucicacid (LEA)B. napuscv.Westar, were amplifiedbyPCRand expressed inyeast cells under the control of the strong galactose-inducible promoter. As expected, yeast cells expressing theFAE1genes from HEA Brassicaspp. synthesized very long chain monounsaturated fatty acids that are not normally found in yeast, while fatty acid profiles of yeast cells expressing theFAE1gene from LEA B. napuswere identical to control yeast samples....

7p tumor12 22-04-2013 41 2   Download

Acomputational approach is used to analyse temporal gene expression in the context of metabolic regulation. It is based on the assumption that cells developed optimal adaptation strategies to changing environmental conditions. Time-dependent enzyme profiles are calculatedwhichoptimize the function of a metabolic pathway under the constraint of limited total enzymeamount.For linearmodel pathways it is shown thatwave-like enzyme profiles are optimal for a rapid substrate turnover.

8p tumor12 22-04-2013 49 3   Download

Thyroid hormone has various effects on cell proliferation, growth and apoptosis. To gain more insight into the molecular dynamics caused by thyroid hormone, gene expression in HeLaTRcells that constitutively over-expressed the thyroid hormone receptor (TR) was analyzed. Gene expression profiling of the HeLaTRcells with an oligonucleotide microarray yielded 229 genes whose expression was significantly altered by T3.

10p fptmusic 16-04-2013 36 4   Download

Numerous innovations in high-throughput protein production and micro-array surface technologies have enabled the development of addressable formats for proteins ordered at high spatial density. Protein array imple-mentations have largely focused on antibody arrays for high-throughput protein profiling. However, it is also possible to construct arrays of full-length, functional proteins from a library of expression clones.

12p fptmusic 12-04-2013 43 4   Download

This report describes an efficient strategy for amplified functional purification of the human H1 receptor after heterologous expression in Sf9 cells. The cDNA encoding a C-terminally histidine-tagged (10xHis) human histamineH1 receptor was used to generate recombinant baculovirus in a Spodoptera frugiperda-derived cell line (IPLB-Sf9). As judged from its ligand affinity profile, functional receptor could be expressed at high levels (30–40 pmol per 10 6 cells).

11p dell39 03-04-2013 60 4   Download

Intracellular heme concentrations are maintained in part by heme degrada-tion, which is catalyzed by heme oxygenase. Heme oxygenase consists of two structurally related isozymes, HO-1 and HO-2. Recent studies have identified HO-2 as a potential oxygen sensor. To gain further insights into the regulatory role of HO-2 in heme homeostasis, we analyzed the expres-sion profiles of HO-2 and the biochemical consequences of HO-2 knock-down with specific short interfering RNA (siRNA) in human cells.

14p inspiron33 23-03-2013 32 4   Download

Over the years, several studies have been performed to analyse plant–patho-gen interactions. Recently, functional genomic strategies, including proteo-mics and transcriptomics, have contributed to the effort of defining gene and protein function and expression profiles. Using these ‘omic’ approaches, pathogenicity- and defence-related genes and proteins expressed during phytopathogen infections have been identified and enor-mous datasets have been accumulated.

16p galaxyss3 07-03-2013 30 3   Download

The role of the ATP-binding cassette ABCB1 in mediating the resistance to chemotherapy in many forms of cancer has been well established. The pro-tein is also endogenously expressed in numerous barrier and excretory tissues, thereby regulating or impacting on drug pharmacokinetic profiles.

10p mobifone23 18-01-2013 45 5   Download

The metallo-b-lactamase (MBL) GOB-1 was expressed via a T7 expression system in Escherichia coliBL21(DE3). The MBL was purified to homoge-neity and shown to exhibit a broad substrate profile, hydrolyzing all the tested b-lactam compounds efficiently.

12p cosis54 05-01-2013 43 4   Download