Genome editing approaches with CRISPR/ Cas9: The association of NOX4 expression in breast cancer patients and effectiveness evaluation of different strategies of CRISPR/ Cas9 to knockout Nox4 in cancer cells

The increasing prevalence of cancer detection necessitated practical strategies to deliver highly accurate, beneficial, and dependable processed information together with experimental results. We deleted the cancer biomarker NOX4 using three novel genetic knockout (KO) methods. Homology-directed repair (HDR), Dual allele HITI (Du-HITI) and CRISPR-excision were utilized in this study.