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Impact of stationary-phase pore size on chromatographic performance using oligonucleotide separation as a model
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A combined experimental and theoretical study was performed to understand how the pore size of packing materials with pores 60–300 A˚ in size affects the separation of 5–50-mer oligonucleotides. For this purpose, we developed a model in which the solutes were described as thin rods to estimate the accessible surface area of the solute as a function of the pore size and solute size.
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