Impacts of culture conditions on ligninolytic enzyme (LIP, MNP, and Lac) activity of five bacterial strains

In this study, with the aim of determining and assessing the influence of several culture conditions on the ligninolytic enzyme (LiP, MnP, and Lac) activity of bacteria, five lignin-degrading bacteria strains were isolated from two different soil samples and cultured on minimum salt medium agar containing alkaline lignin (MSML agar). Among the five isolated strains, DL1 and X3 expressed strong and stable ligninase enzyme activity at various temperature levels (30C, 37C, 50C, and 60C) and were selected for further study.