Indirect regeneration of japonica rice (Oryza sativa L.) varieties through anther culture

Effect of growth regulators on frequency of callus induction and shoot regeneration from anthers of two japonica rice varieties viz., Azucena and Moroberekan were studied. Anthers from panicles in which the distance between flag leaf and subtending leaf was 12- 13 cm in Azucena and 14-15 cm in Moroberekan were selected for culture.