Multiplexed immunofluorescence analysis of CAF-markers, EZH2 and FOXM1 in gastric tissue: Associations with clinicopathological parameters and clinical outcomes

The aim of this study is to explore the expression and clinical relevance of CAF-associated markers, EZH2 and FOXM1 in gastric samples. Protein expression were detected and evaluated by multi-plex immunofuorescence (mIF) in 93 cases of gastric cancer (GC) and 31 cases of gastric intraepithelial neoplasia (GIN). The correlation among their expression, and the relationship of them with clinicopathological parameters and prognosis in GC were then analyzed.