Optimization of culture conditions using one-factor-at-time methodology and partial purification of amylase from aspergillus niger of DTO: H5 under solid state fermentation

The aim of this study was to isolate amylase-producing fungi, optimize the cultural conditions using solid state fermentation (SSF) and characterize the partially purified enzyme. Six fungal strains isolated from soil and decayed onion samples were screened for their ability to secrete amylase. Culture medium was optimized using One-factor-at-aTime (OFAT) methodology under SSF.