Strategy to identify reduced arabinoxylo-oligosaccharides by HILIC-MS

Identification of arabinoxylo-oligosaccharides (AXOS) within complex mixtures is an ongoing analytical challenge. Here, we established a strategy based on hydrophilic interaction chromatography coupled to collision induced dissociation-mass spectrometry (HILIC-MSn ) to identify a variety of enzyme-derived AXOS structures.