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2-D gel electrophoresis
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Ebook "11th International conference on practical applications of computational biology & bioinformatics" research are increasingly driven by experimental techniques that challenge our ability to analyse, process and extract meaningful knowledge from the underlying data. The impressive capabilities of next-generation sequencing technologies, together with novel and constantly evolving, distinct types of omics data technologies, have created an increasingly complex set of challenges for the growing fields of Bioinformatics and Computational Biology.
345p
lucchinguyen
28-12-2023
3
2
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Propofol is a safe and effective intravenous anesthetic that is widely used for the induction and maintenance of anesthesia during surgery. However, the mechanism by which propofol exerts its anesthetic effect remains unknown. The rapid onset of phosphorylation modifications coincides with that of propofol anesthesia.
10p
viisaacnewton
26-04-2022
7
1
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A series of novel positional oximes (2a–2d), O-methyl oxime ethers (3a–3d), and O-benzyl oxime ethers (4a–4d) were synthesized in high yields starting from their corresponding methyl 3-, 4-, 6-, and 13-keto tetradecanoates. The synthesized compounds were characterized by 1H NMR, 13C NMR, FT-IR, mass, and elemental analyses for their structures and (E) /(Z) isomerizations. Their DNA binding abilities were investigated in vitro by agarose gel electrophoresis. The antibacterial and antifungal activities were tested also in vitro against eleven bacterial strains and three fungal strains.
14p
langthannam
29-12-2021
8
1
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Although the pathogenesis of adolescent idiopathic scoliosis (AIS) remains unclear, there are little evidences of the pathogenesis in patients with thoracolumbar/lumbar AIS. The purpose of this study was to identify proteins or proteomes that may be causally related to the pathogenesis of AIS with structured thoracolumbar/ lumbar curvature using two-dimensional fluorescence difference gel electrophoresis (2D-DIGE).
8p
vivermont2711
28-01-2021
11
2
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Two-dimensional polyacrylamide gel electrophoresis (2D PAGE) is commonly used to identify differentially expressed proteins under two or more experimental or observational conditions.
14p
viwyoming2711
16-12-2020
18
1
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Two-dimensional gel electrophoresis (2DE) is a powerful technique for studying protein isoforms and their modifications. Existing commercial 2D image analysis tools rely on spot detection that limits analysis of complex protein profiles, e.g. spot appearance/disappearance or overlapping spots.
5p
viwyoming2711
16-12-2020
17
2
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Two-dimensional differential gel electrophoresis (2D-DIGE) provides a powerful technique to separate proteins on their isoelectric point and apparent molecular mass and quantify changes in protein expression. Abundantly available proteins in spots can be identified using mass spectrometry-based approaches.
11p
vikentucky2711
26-11-2020
15
1
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The analysis of abiotic stress responsiveness in plants is a very crucial way to identify the genes conferring stress tolerance and their use in crop improvement programme. Heat stress is one of the stresses which highly affect the growth, quality character as well as total yield of the crop. In this study, a proteome analysis by 2D electrophoresis was used to identify the characteristic change in two wheat genotypes viz, GW 451 (heat tolerant) and WH 147 (heat susceptible) from flag leaf.
8p
kethamoi4
16-04-2020
13
2
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Proteomics is becoming an increasingly important tool for the study of many different aspects of plant functions, such as investigating the molecular processes underlying hostpathogen interaction, plant physiology, development and differentiation. Cassava mosaic disease (CMD), caused by cassava mosaic virus (CMV), is the most serious disease in cassava. However, the molecular mechanisms underlying CMD in cassava during CMV infection is not yet clearly understood.
18p
quenchua2
15-12-2019
23
0
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Therehas longbeenconjectureover thedegree towhichthere may be structural and functional heterogeneity in the tetra-mericserineproteasetryptase(EC 3.4.21.59), amajor mediator of allergic inflammation. We have applied 2D gel electrophoresis to analyze the extent, nature, and variability of thisheterogeneity inlysatesofmast cells isolatedfromlung and skin, and in preparations of purified tryptase. Gels were silver stained, or the proteins transferred to nitrocellulose blots and probed with either tryptase-specific monoclonal antibodies or various lectins. ...
14p
tumor12
20-04-2013
36
2
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Hemocyanins are large oligomeric copper-containing pro-teins that serve for the transport of oxygen in many arth-ropod species. While studied in detail in the Chelicerata and Crustacea, hemocyanins had long been considered unnec-essary in the Myriapoda. Here we report the complete molecular structure of the hemocyanin from the common house centipedeScutigera coleoptrata(Myriapoda: Chilo-poda), as deduced from 2D-gel electrophoresis, MALDI-TOF mass spectrometry, protein and cDNA sequencing, and homology modeling....
9p
fptmusic
16-04-2013
34
2
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Two thioredoxin h isoforms, HvTrxh1 and HvTrxh2, were identified in two and one spots, respectively, in a proteome analysis of barley (Hordeum vulgare) seeds based on 2D gel electrophoresis and MS. HvTrxh1 was observed in 2D gel patterns of endosperm, aleurone layer and embryo of mature barley seeds, andHvTrxh2was presentmainly in the embryo. During germination, HvTrxh2 decreased in abun-dance and HvTrxh1 decreased in the aleurone layer and endospermbut remainedat high levels in the embryo.
11p
fptmusic
16-04-2013
40
1
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Neural stimulation controls the contractile properties of skeletal muscle fibres through transcriptional regulation of a number of proteins, including myosin isoforms. To study whether neural stimulation is also involved in the control of post-translational modifications of myosin, we analysed the phosphorylation of alkali myosin light chains (MLC1) and regulatory myo-sin light chains (MLC2) in rat slow (soleus) and fast (extensor digitorum longus EDL) muscles using 2D-gel electrophoresis and mass spectrometry....
15p
dell39
27-03-2013
50
4
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The major potato tuber proteins of the Kuras cultivar, which is the domin-ant cultivar used in Northern Europe for industrial starch production, were analysed using 1D and 2D gel electrophoresis. The electrophoretic patterns varied significantly depending on the method of preparation and the potato variant (Solanum tuberosum).
16p
inspiron33
25-03-2013
38
3
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Ten genes from two multigene families encode barleya-amylases. To gain insight into the occurrence and fate of individual isoforms during seed ger-mination, thea-amylase repertoire was mapped by using a proteomics approach consisting of 2D gel electrophoresis, western blotting, and mass spectrometry.
14p
galaxyss3
21-03-2013
50
2
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After screening 25 marine invertebrates, a novel metallocarboxypeptidase (SmCP) has been identified by activity and MS analytical approaches, and isolated from the marine annelid Sabellastarte magnifica. The enzyme, which is a minor component of the molecularly complex animal body, as shown by 2D gel electrophoresis, has been purified from crude extracts to homogeneity by affinity chromatography on potato carboxypeptidase inhib-itor and by ion exchange chromatography.
16p
vinaphone15
25-02-2013
34
1
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A higher prevalence ofCampylobacter concisusand higher levels of IgG antibodies specific toC. concisusin Crohn’s disease patients than in con-trols were recently detected. In this study, 1D and 2D gel electrophoresis coupled with LTQ FT-MS and QStar tandem MS, respectively, were per-formed to characterize the secretome of a C.
12p
mobifone23
18-01-2013
42
2
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The gold standard of assessing liver fibrosis is liver biopsy, which is invasive and not without risk. Therefore, searching for noninvasive serologic biomarkers for liver fibrosis is an importantly clinical issue. Methods: A total of 16 healthy volunteers and 45 patients with chronic hepatitis C virus (HCV) were enrolled (F0: n = 16, F1: n = 7, F2: n = 17, F3: n = 8 and F4: n = 13, according to the METAVIR classification). Three serum samples of each fibrotic stage were analyzed by two-dimension difference gel electrophoresis (2D-DIGE). The differential proteins were identified by...
7p
toshiba23
18-11-2011
53
3
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The antiproliferative effect of the Hsp90 inhibitor 17-AAG (17-allylamino-17-demethoxygeldanamycin) on human retinal pigment epithelial cells is investigated. Methods: MTT and flow cytometry were used to study the antiproliferative effects of the 17-AAG treatment of ARPE-19 cells. 2D gel electrophoresis (2-DE) and mass spectrometry were applied to detect the altered expression of proteins, which was verified by real-time PCR.
15p
toshiba23
18-11-2011
42
2
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