3D spheroids

In this study, human induced pluripotent stem cells (hiPSCs) were successfully differentiated into cardiomyocytes and subsequently used to generate spheroids via the hanging drop method. We comprehensively characterized the formation, fusion, and beating capability of the generated spheroids.

11p viambani 18-06-2024 1 0   Download

In this research, we fabricated α-mangostin-loaded liposomes from soybean lecithin and cholesterol to enhance the solubility in water of α-mangostin and reduce side effects in the treatment of cancer.

11p viamancio 04-06-2024 2 0   Download

Angiogenesis is an important hallmark of Glioblastoma (GBM) marked by elevated vascular endothelial growth factor-A (VEGF-A) and its receptor 2 (VEGFR-2). As previously reported nimbolide (NBL), trans-chalcone (TC) and piperine (PPR) possess promising antiangiogenic activity in several cancers however, their comparative efcacy and mechanism of antiangiogenic activity in GBM against VEGFR-2 has not been elucidated.

21p vileonardodavinci 23-12-2023 3 2   Download

Recapitulating the tumor microenvironment (TME) in vitro remains a major hurdle in cancer research. In recent years, there have been significant strides in this area, particularly with the emergence of 3D spheroids as a model system for drug screening and therapeutics development for solid tumors. However, incorporating macrophages into these spheroid cultures poses specific challenges due to the intricate interactions between macrophages and cancer cells.

17p vileonardodavinci 23-12-2023 4 2   Download

Drug testing in vitro cell culture models more accurately would be of significant value to the medical field and pharmaceutical industry. To achieve this goal, microfluidic cell culture platforms are created and improved for modeling the native cell microenvironment because they can precisely reconstruct in vivo cellular behavior.

10p viannee 02-08-2023 6 3   Download

In this study, it was aimed to obtain 3D scaffolds using medical grade chitosan (MC) with a high deacetylation degree (DD ≥ 92.6%) to overcome the spheroid formation of rat adipose tissue derived mesenchymal stem cells (rAdMSCs) on control chitosan (C, DD = 75%–85%) scaffolds. Genipin was used as a biological chemical crosslinker, and glycerol phosphate salt was used both as a pH adjusting agent and physical crosslinker.

13p lyhuyenthu 31-01-2023 14 2   Download

Nghiên cứu tiến hành khảo sát tác động của cao chiết gừng được chiết xuất bằng ba phương pháp: ngâm dầm (G1), Soxhlet (G3) và siêu âm (G5) lên tế bào ung thư gan HepG2 nuôi cấy dạng 3D. Sự tác động của cao chiết gừng trên tế bào HepG2 được đánh giá bằng các phương pháp đánh giá hình thái spheroid, xác định đường cong tăng trưởng, xác định tỷ lệ tế bào chết.

4p viclerkmaxwel 16-02-2022 13 2   Download

The mechanisms of action and efficacy of cisplatin and paclitaxel at cell population level are well studied and documented, however the localized spatio-temporal effects of the drugs are less well understood. We explore the emergence of spatially preferential drug efficacy resulting from variations in mechanisms of cell-drug interactions.

16p vichaeng2711 29-04-2021 9 1   Download

Pancreatic ductal adenocarcinoma (PDAC) is the fourth most common cause of cancer related death. It is lethal in nearly all patients, due to an almost complete chemoresistance. Most if not all drugs that pass preclinical tests successfully, fail miserably in the patient.

13p vijisoo2711 29-09-2020 8 1   Download

The complex cellular networks within tumors, the cytokine milieu, and tumor immune escape mechanisms affecting infiltration and anti-tumor activity of immune cells are of great interest to understand tumor formation and to decipher novel access points for cancer therapy.

13p vialabama2711 21-09-2020 14 1   Download

Dispersal of glioblastoma (GBM) cells leads to recurrence and poor prognosis. Accordingly, molecular pathways involved in dispersal are potential therapeutic targets. The mitogen activated protein kinase/extracellular signal regulated kinase (MAPK/ERK) pathway is commonly dysregulated in GBM, and targeting this pathway with MEK inhibitors has proven effective in controlling tumor growth.

11p vimale2711 21-08-2020 9 1   Download

Different 3D-cell culture approaches with varying degrees of complexity have been developed to serve as melanoma models for drug testing or mechanistic studies.

14p vikuala271 13-06-2020 6 0   Download

Bronchial carcinoids are neuroendocrine tumors that present as typical (TC) and atypical (AC) variants, the latter being more aggressive, invasive and metastatic.

19p vikuala271 13-06-2020 13 1   Download

Pancreatic ductal adenocarcinoma is a devastating disease with poor outcome, generally characterized by an excessive stroma component. The purpose of this study was to develop a simple and reproducible in vitro 3D assay employing the main constituents of pancreatic ductal adenocarcinoma, namely pancreatic stellate and cancer cells.

13p viorochimaru2711 01-06-2020 11 1   Download

Three-dimensional (3D) spheroids are frequently used in toxicological study because their morphology and function closely resemble those of tissue. As these properties are maintained over a long term, repeated treatment of the spheroids with a test object is possible.

12p vihamax2711 21-04-2020 15 0   Download

α-Mangostin (αMG) is extracted from Garcinia mangostana Linn and exerts antiproliferative activities. Although several researches on αMG were performed using cell monolayers, the in vitro pharmacological effects on 3D cancer models have never been investigated.

8p vinasaki2711 12-11-2019 11 0   Download