Allergens

Bài viết trình bày khảo sát mối liên quan giữa kết quả xét nghiệm Immunoglobin E đặc hiệu dị nguyên (allergen-specific Immunoglobin E - sIgE) với một số đặc điểm lâm sàng cận lâm sàng bệnh nhân mày đay mạn tính, từ đó đánh giá vai trò của IgE đặc hiệu dị nguyên trong chẩn đoán căn nguyên mày đay mạn tính.

5p vicarlos 21-05-2024 10 3   Download

Bài viết IGE đặc hiệu với 52 dị nguyên và mối liên quan với một số đặc điểm lâm sàng, cận lâm sàng trên bệnh nhân mày đay mạn tính mô tả kết quả và khảo sát mối liên quan của Immunoglobin E (IgE) đặc hiệu với 52 dị nguyên (allergen-specific IgE - sIgE) và mối liên quan với một số đặc điểm lâm sàng cận lâm sàng bệnh nhân mày đay mạn tính.

7p vidudley 20-02-2023 7 2   Download

Twelve volatile constituents were identified from Liverwort Porella densifolia (Hepaticae) collected at Ham Rong Mountain, Sapa, Lao Cai Province by gas chromatography/mass spectrometry (GC/MS). The volatile constituents including some monoterpenes (β-myrcene, α- pinene and camphene); two major sesquiterpenes, deoxopinguisone and bicyclogermacrene-2 were identified.

4p larachdumlanat127 20-12-2020 6 2   Download

Liệu pháp miễn dịch đặc hiệu dị nguyên (AIT: allergen specific immunotherapy) đang là phương pháp duy nhất điều trị bệnh dị ứng đầy tiềm năng. Liệu pháp này bao gồm việc sử dụng liều cao và lặp lại các chất gây dị ứng bằng đường tiêm dưới da (SCIT: subcutaneous immunotherapy) hoặc dùng ngậm dưới lưỡi (SLIT: sublingual immunotherapy), để tạo ra trạng thái dung nạp miễn dịch lâu dài.

14p viamsterdam 17-09-2020 38 1   Download

Sensitization to cockroach allergens is associated with the development of asthma. Bla g 1 is a German cockroach allergen that shows allergenic cross-reactivity with American cockroach allergen, Per a 1, and has a molecular structure composed of multiple tandem amino-acid repeats. Two consecutive repeats are not identical but form a duplex that constitutes a basic molecular unit of Bla g 1. By molecular mass, purified natural Bla g 1 would contain approximately two duplexes.

7p system191 01-06-2013 44 4   Download

Expansins are a family of proteins that catalyze pHdependent long-term extension of isolated plant cell walls. They are divided into two groups, a and b, the latter consisting of the grass group I pollen allergens and their vegetative homologs. Expansins are suggested to mediate plant cell growth by interfering with either structural proteins or the polysaccharide network in the cell wall.

10p research12 01-06-2013 28 4   Download

The mature cysteine protease from Dermatophgoides pteronyssinus, Der p 1, is a major house dust mite allergen. Its enzymatic activity has been shown to have pro-in¯am-matory e€ects that could alsonegatively in¯uence ecacy of allergen-speci®c immunotherapy. The aimof this study was to express recombinant pro-Der p 1 (rpro-Der p 1) in the yeastPichia pastorisand to study itsmaturation.

9p research12 23-04-2013 30 2   Download

Until now, only a small amount of information is available about tomato allergens. In the present study, a glycosylated allergen of tomato (Lycopersicon esculentum), Lyc e 2, was purified from tomato extract by a two-step FPLC method. The cDNA of two different isoforms of the protein, Lyc e 2.01 and Lyc e 2.02, was cloned into the bacterial expressionvector pET100D. The recombinant proteinswere purified by electroelution and refolded.

11p tumor12 20-04-2013 32 3   Download

We have previously cloned, expressedand characterized two variants of the major allergen Lep d 2 from culturedLepi-doglyphus destructor mites. These variants, Lep d 2.0101 and Lep d 2.0201, differ at 13 amino acid positions. In this study we investigated Lep d 2 sequence diversity between wild and cultured mites. PCR, Southern blot and DNA sequence analysis revealed the presence of two different Lep d 2 genes, one with and one without an intron.

8p tumor12 20-04-2013 47 3   Download

Wheatx-5 gliadin has been identified as a major allergen in wheat-depend-ent exercise-induced anaphylaxis. We have detected seven IgE-binding epitopes in primary sequence of the protein. We newly identified four additional IgE-binding epitope sequences, QQFHQQQ, QSPEQQQ, YQQYPQQ and QQPPQQ, in three patients with wheat-dependent exer-cise-induced anaphylaxis in this study.

8p fptmusic 11-04-2013 40 2   Download

Hyaluronidase (E.C. 3.2.1.35), one of the three major allergens of yellow jacket venom, is a glycoprotein of 45 kDa that is largely responsible for the cross-reactivity of wasp and bee venoms with sera of allergic patients. The asparagine-linked carbohydrate often appears to constitute the com-mon IgE-binding determinant.

9p fptmusic 11-04-2013 44 4   Download

Inhaled environmental antigens, i.e. allergens, cause allergic symptoms in millions of patients worldwide. As little is known about the fate of an aller-gen upon inhalation, we addressed this issue for a major dust mite allergen, Der p 2. First, a model for Der p 2-sensitization was established in C57BL⁄6 J mice, in which sensitized mice mounted a Der p 2-specific IgE-response with eosinophilic lung inflammation after allergen challenge in the airways.

12p fptmusic 11-04-2013 41 1   Download

Aspergillus fumigatusis responsible for many allergic respiratory diseases, the most notable of which )due to its severity)is allergic bronchopulmo-nary aspergillosis. Aspf1 is a major allergen of this fungus: this 149-amino acid protein belongs to the ribotoxin family, whose best characterized member isa-sarcin (EC 3.1.27.10). The proteins of this group are cytotoxic ribonucleases that degrade a unique bond in ribosomal RNA impairing protein biosynthesis.

9p awards 06-04-2013 37 3   Download

The major 2S albumin allergen from Brazil nuts, Ber e 1, was subjected to gastrointestinal digestion using a physiologically relevantin vitro model sys-tem either before or after heating (100
C for 20 min). Whilst the albumin was cleaved into peptides, these were held together in a much larger struc-ture even when digested by using a simulated phase 1 (gastric) followed by a phase 2 (duodenal) digestion system. Neither prior heating of Ber e 1 nor the presence of the physiological surfactant phosphatidylcholine affected the pattern of proteolysis.

0p awards 05-04-2013 41 2   Download

Approximately 400 million allergic patients are sensitized against group 1 grass pollen allergens, a family of highly cross-reactive allergens present in all grass species. We report the eukaryotic expression of the group 1 aller-gen from Timothy grass, Phl p 1, in baculovirus-infected insect cells. Domain elucidation by limited proteolysis and mass spectrometry of the purified recombinant glycoprotein indicates that the C-terminal 40% of Phl p 1, a major IgE-reactive segment, represents a stable domain....

0p awards 05-04-2013 27 2   Download

Ag-NPA-1 (AgFABP), a 15 kDa lipid binding protein (LBP) fromAscari-dia galli, is a member of the nematode polyprotein allergen/antigen (NPA) family. Spectroscopic analysis shows that Ag-NPA-1 is a highly ordered, a-helical protein and that ligand binding slightly increases the ordered sec-ondary structure content. The conserved, single Trp residue (Trp17) and three Tyr residues determine the fluorescence properties of Ag-NPA-1.

10p awards 05-04-2013 59 6   Download

Malassezia sympodialisis an opportunistic yeast that col-onizes human skin and may induce IgE and T cell reac-tivity in patients with atopic eczema/dermatitis syndrome (AEDS). Previously, we have cloned and expressed six recombinant allergens (rMala s 1 and rMala s 5 to rMal-a s 9) from this yeast. By combining high throughput screening and phage surface display techniques, 27 com-plete and partial IgE-binding clones of M. sympodialis have been identified. Here we enlarged the panel of recombinant M.

10p dell39 03-04-2013 40 3   Download

Bermuda grass pollen (BGP) contains a very complex mixture of allergens, but only a few have been characterized. One of the allergens, with an apparent molecular mass of 21 kDa, has been shown to bind serum IgE from 29% of patients with BGP allergy. A combination of chromato-graphic techniques (ion exchange and reverse phase HPLC) was used to purify the 21 kDa allergen.

10p dell39 27-03-2013 60 3   Download

Employing transgenic plants as alternative systems to the conventional Escherichia coli, Pichia pastorisor baculovirus hosts to produce recombin-ant allergens may offer the possibility of having available edible vaccines in the near future. In this study, two EF-hand-type Ca 2+ -binding allergens from olive pollen, Ole e 3 and Ole e 8, were produced in transgenic Arabid-opsis thaliana plants.

10p inspiron33 25-03-2013 40 3   Download

Par j 1 and Par j 2 proteins are the two major allergens inParietaria juda-ica pollen, one of the main causes of allergic diseases in the Mediterranean area. Each of them contains eight cysteine residues organized in a pattern identical to that found in plant nonspecific lipid transfer proteins.

14p vinaphone15 27-02-2013 48 4   Download