Báo cáo khoa học: Cloning, expression and characterization of two new IgE-binding proteins from the yeast Malassezia sympodialis with sequence similarities to heat shock proteins and manganese superoxide dismutase
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Malassezia sympodialisis an opportunistic yeast that col-onizes human skin and may induce IgE and T cell reac-tivity in patients with atopic eczema/dermatitis syndrome (AEDS). Previously, we have cloned and expressed six recombinant allergens (rMala s 1 and rMala s 5 to rMal-a s 9) from this yeast. By combining high throughput screening and phage surface display techniques, 27 com-plete and partial IgE-binding clones of M. sympodialis have been identified. Here we enlarged the panel of recombinant M. sympodialisallergens by RACE–PCR, cloning and nucleotide sequencing to obtain the coding sequences of two new IgE-binding clones. ...
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