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Báo cáo khoa học: Cloning, expression and characterization of a family-74 xyloglucanase from Thermobifida fusca
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Thermobifida fusca xyloglucan-specific endo-b-1,4-gluca-nase (Xeg)74 and the Xeg74 catalytic domain (CD) were cloned, expressed inEscherichia coli, purified and charac-terized.This enzymehas aglycohydrolase family-74CDthat is a specific xyloglucanase followed by a family-2 carbo-hydrate binding module at the C terminus.The Michaelis constant (Km) andmaximal rate (Vmax) values for hydrolysis of tamarind seed xyloglucan (tamXG) are 2.4lMand 966lmol xyloglucan oligosaccharides (XGOs) min )1 Ælmol protein )1 ....
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