Analysis of carotenoids

An analytical procedure for the analysis of carotenoids in marine sediments rich in organic matter has been developed. Analysis of these compounds is difficult; the application of methods used by other authors required optimization for the samples studied here.

14p tocectocec 25-05-2020 28 1   Download

The Phycomyces blakesleeanus wild-type is yellow, because it accumulates b-carotene as the main carotenoid. A new carotenoid mutant of this fungus (A486) was isolated, after treatment with ethyl methane sulfonate (EMS), showing a whitish coloration. It accumulates large amounts of phytoene, small quantities of phyto¯uene, f-carotene and neurosporene, in decreasing amounts, and traces of b-carotene. This phenotype indicates that it carries a leaky mutation a€ecting the enzyme phytoene dehydrogenase (EC 1.3.-.-), which is speci®ed by the gene carB....

7p research12 01-06-2013 48 5   Download

Mycobacterium tuberculosis, the causative agent of tuberculosis, is assumed to lack carotenoids, which are widespread pigments fulfilling important functions as radical scavengers and as a source of apocarotenoids. In mam-mals, the synthesis of apocarotenoids, including retinoic acid, is initiated by theb-carotene cleavage oxygenases I and II catalyzing either a central or an excentric cleavage of b-carotene, respectively.

12p viettel02 19-02-2013 30 2   Download