Binding site prediction

The functional site ofÔphospholipase A2 inhibitor from pythonÕ (PIP) was predicted based on the hypothesis of proline brackets. Using di€erent sources of secretory phospholipase A2(sPLA2 s) as enzyme, and [ 3 H]arachido-nate-labelledEscherichia colias substrate, short synthetic peptides representing the proposed site were examined for their secretoryphospholipase A2(sPLA2 ) inhibitoryactivity.

9p research12 23-04-2013 43 2   Download

CYP73 enzymes are highly conserved cytochromes P450 in plant species that catalyse the regiospecific 4-hydroxylation ofcinnamicacid to formprecursorsofligninandmanyother phenolic compounds. A CYP73A1 homology model based on P450 experimentally solved structures was used to iden-tify active site residues likely togovern substrate binding and regio-specific catalysis. The functional significance of these residues was assessed using site-directedmutagenesis.

12p tumor12 20-04-2013 40 3   Download

Ech hydrogenase fromMethanosarcina barkeriis a member of a distinct group of membrane-bound [NiFe] hydrogenases with sequence similarity to energy-conserving NADH:quinone oxidoreductase (complex I). The sequence of the enzyme predicts the binding of three [4Fe-4S] clusters, one by subunit EchC and two by subunit EchF. Previous studies had shown that two of these clusters could be fully reduced under 10 5 Pa of H2at pH 7 giving rise to two distinct S½ electron paramagnetic resonance (EPR) signals, designated as the g¼1.89 and theg¼1.92 signal....

13p fptmusic 12-04-2013 36 2   Download

DNA-binding sites for SYCRP1, which is a regulatory protein of the cyanobacterium Synechocystissp. PCC6803, were predicted for the whole genome sequence by estimating changes in the binding free energy (DDG A total ) for SYCRP1 for those sites. The DDG A total values were calculated by summingDDGvalues derived from systematic single base-pair substitu-tion experiments (symmetrical and cooperative binding model).

10p inspiron33 23-03-2013 39 4   Download

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học quốc tế cung cấp cho các bạn kiến thức về ngành y đề tài: Protein-lipid interactions: correlation of a predictive algorithm for lipid-binding sites with three-dimensional structural data

14p toshiba17 31-10-2011 49 3   Download