Blood culture

In this study, we tested the ability of a specific culture medium supplemented with a cytokine cocktail to increase the number of total MNCs and thereby enrich CD34+UCHSCs. In addition, we investigated the effects of the bioactive peptide SL-13R on the proliferation of CD34+UC-HSCs at different culture time points and peptide doses.

7p xuanphongdacy06 18-09-2024 4 1   Download

India accounts for 30% of neonatal deaths globally. Bacterial sepsis is a significant cause of morbidity and mortality in newborns. The study helps to make antibiotic policy in neonatal sepsis. The main objective is to study the incidence of multidrug resistant gram negative and gram positive organisms causing neonatal septicemia and their antibiotic sensitivity pattern. The study was conducted in the Department of Microbiology over a period of one year. Sample of blood was collected under aseptic precautions and processed by standard techniques.

5p gaocaolon5 14-06-2020 24 3   Download

Human colon adenocarcinoma cells (HT29-ATCC) and the clone HT29-5F7 were cultured under conditions that differentiate cells to a polarized intestinal phenotype. Differentiated cells showed the presence of junctional complexes and intercellular lumina bordered by microvilli. Intestinal brush border hydrolase activities (sucrase, aminopeptidase N, lactase and mal-tase) were detected mainly in differentiated HT29-ATCC cells compared with the differentiated clone, HT29-5F7.

10p inspiron33 25-03-2013 37 3   Download

Endothelial cells are indispensable components of the vascular system, and play pivotal roles during development and in health and disease. Their properties have been studied extensively byin vivo analysis of genetically modified mice.

11p media19 06-03-2013 56 3   Download

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Functional characterization of human Cd33+ And Cd11b+ myeloid-derived suppressor cell subsets induced from peripheral blood mononuclear cells co-cultured with a diverse set of human tumor cell lines

20p dauphong6 04-01-2012 60 7   Download

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài:Effect of anti-CD52 antibody alemtuzumab on ex-vivo culture of umbilical cord blood stem cells

10p toshiba24 06-12-2011 51 5   Download

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học 'Respiratory Research cung cấp cho các bạn kiến thức về ngành y đề tài: "The role of IFN-γ in regulation of IFN-γ-inducible protein 10 (IP-10) expression in lung epithelial cell and peripheral blood mononuclear cell co-cultures...

11p toshiba12 21-10-2011 44 4   Download

Infection with Viridans Streptococci: Treatment Isolates from neutropenic patients with bacteremia are often resistant to penicillin; thus these patients should be treated presumptively with vancomycin until the results of susceptibility testing become available. Viridans streptococci isolated in other clinical settings usually are sensitive to penicillin. Abiotrophia Species (Nutritionally Variant Streptococci) Occasional isolates cultured from the blood of patients with endocarditis fail to grow when subcultured on solid media.

5p colgate_colgate 21-12-2010 88 4   Download

Antimicrobial Therapy for Selected Settings For uncomplicated skin and soft tissue infections, the use of oral antistaphylococcal agents is usually successful. For other infections, parenteral therapy is indicated. S. aureus endocarditis is usually an acute, life-threatening infection. Thus prompt collection of blood for cultures must be followed immediately by empirical antimicrobial therapy. For S. aureus native-valve endocarditis, a combination of antimicrobial agents is often used.

7p colgate_colgate 21-12-2010 62 3   Download

Diagnosis While the detection of CoNS at sites of infection or in the bloodstream is not difficult by standard microbiologic culture methods, interpretation of these results is frequently problematic. Since these organisms are present in large numbers on the skin, they often contaminate cultures. It has been estimated that only 10–25% of blood cultures positive for CoNS reflect true bacteremia. Similar problems arise with cultures of other sites. Among the clinical findings suggestive of true bacteremia are fever, evidence of local infection (e.g.

4p colgate_colgate 21-12-2010 60 4   Download

Kingella kingae Because of improved microbiologic methodology, isolation of K. kingae is increasingly common. Inoculation of clinical specimens (e.g., synovial fluid) into aerobic blood culture bottles enhances recovery of this organism. In recent series, K. kingae has been the third most common cause of septic arthritis in children

5p colgate_colgate 21-12-2010 81 3   Download

Other Moraxella Species Other Moraxella species are occasional causes of a wide range of infections, including bronchitis, pneumonia, empyema, endocarditis, meningitis, conjunctivitis, endophthalmitis, urinary tract infection, septic arthritis, and wound infection. In a report on all Moraxella isolates submitted to the Centers for Disease Control and Prevention between 1953 and 1980, certain clinical associations were apparent (Table 138-2). M. osloensis and M.

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Diagnosis The diagnosis of clostridial disease, in association with positive cultures, must be based primarily on clinical findings. Because of the presence of clostridia in many wounds, their mere isolation from any site, including the blood, does not necessarily indicate severe disease. Smears of wound exudates, uterine scrapings, or cervical discharge may show abundant large gram-positive rods as well as other organisms. Cultures should be placed in selective media and incubated anaerobically for identification of clostridia.

9p colgate_colgate 21-12-2010 62 4   Download

Primary Bacterial Peritonitis: Treatment Treatment for PBP is directed at the isolate from blood or peritoneal fluid. Gram's staining of peritoneal fluid often gives negative results in PBP. Therefore, until culture results become available, therapy should cover gram-negative aerobic bacilli and gram-positive cocci. Third-generation cephalosporins such as cefotaxime (2 g q8h, administered IV) provide reasonable initial coverage in moderately ill patients. Broad-spectrum antibiotics, such as penicillin/β-lactamase inhibitor combinations (e.g., piperacillin/tazobactam, 3.

5p thanhongan 07-12-2010 81 4   Download

The roles of bacteremia and echocardiographic findings in the diagnosis of endocarditis are appropriately emphasized in the Duke criteria. The requirement for multiple positive blood cultures over time is consistent with the continuous low-density bacteremia characteristic of endocarditis (≤100 organisms/mL). Among patients with untreated endocarditis who ultimately have a positive blood culture, 95% of all blood cultures are positive; in 98% of these cases, one of the initial two sets of cultures yields the microorganism.

5p thanhongan 07-12-2010 62 3   Download

Laboratory Investigation The laboratory investigation of patients with lymphadenopathy must be tailored to elucidate the etiology suspected from the patient's history and physical findings. One study from a family practice clinic evaluated 249 younger patients with "enlarged lymph nodes, not infected" or "lymphadenitis." No laboratory studies were obtained in 51%. When studies were performed, the most common were a complete blood count (CBC) (33%), throat culture (16%), chest x-ray (12%), or monospot test (10%).

5p konheokonmummim 03-12-2010 88 3   Download

Endophthalmitis This occurs from bacterial, viral, fungal, or parasitic infection of the internal structures of the eye. It is usually acquired by hematogenous seeding from a remote site. Chronically ill, diabetic, or immunosuppressed patients, especially those with a history of indwelling IV catheters or positive blood cultures, are at greatest risk for endogenous endophthalmitis. Although most patients have ocular pain and injection, visual loss is sometimes the only symptom.

5p ongxaemnumber1 29-11-2010 95 6   Download

Orbital Cellulitis This causes pain, lid erythema, proptosis, conjunctival chemosis, restricted motility, decreased acuity, afferent pupillary defect, fever, and leukocytosis. It often arises from the paranasal sinuses, especially by contiguous spread of infection from the ethmoid sinus through the lamina papyracea of the medial orbit. A history of recent upper respiratory tract infection, chronic sinusitis, thick mucous secretions, or dental disease is significant in any patient with suspected orbital cellulitis. Blood cultures should be obtained, but they are usually negative.

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Question 1: What environment does virus culture require? 1. Nutrient-rich, sterile agar medium. 2. Broth medium containing red blood cells. 3. The environment has sterile, receptor cells. 4. The environment must be sterile and have appropriate pH. 5. Environment has growth substances.

16p womanhood911_07 04-11-2009 7119 6105   Download