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Both enzyme systems
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Vitamins are regarded as vital growth substances. They act as cofactors of various key enzymes involved in metabolism and also have vital influence on metabolic activities of plants. They are believed to play a significant role in plants against abiotic stresses, particularly during water stress. The current experiment was conducted to evaluate the role of exogenously applied thiamin on two white clover cultivars, Super Late Fsd and Layalpur Late, at varying [100% (control), 80%, and 60% field capacities] water regimes.
9p
tudichquannguyet
29-11-2021
17
0
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GmrSD is a modification-dependent restriction endonuclease that specifically targets and cleaves glucosylated hydroxymethylcytosine (glc-HMC) modified DNA. It is encoded either as two separate single-domain GmrS and GmrD proteins or as a single protein carrying both domains. Previous studies suggested that GmrS acts as endonuclease and NTPase whereas GmrD binds DNA.
16p
vioklahoma2711
19-11-2020
9
0
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Immobilized polyextremophilic α-Amylase of Bacillus mycoides for citric acid production using starch
Immobilization is a wide spread technique in the last three decades. It is a powerful method which can improve properties of enzymes like stability, activity, resistance to inhibition due to the by-products in fermentation and its quick recovery. In the present investigation a halophilic organism Bacillus mycoides producing α-amylase was isolated from saline water. A mixed fermenter system was made for citric acid production using starch.
11p
trinhthamhodang1212
06-04-2020
11
1
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Aim of the present work was to study the species composition of fungi found in some thermal springs of Azerbaijan, and to evaluate them according to their activity of hydrolytic enzymes. This study shows that in the formation of mycobiota of thermal water sources of Azerbaijan, located in Greater Caucasus, takes part 22 species of fungi, 77.3% of which belongs to the division of Ascomycota, the others belongs to the division of Zygomycota.
5p
trinhthamhodang3
22-02-2020
11
0
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A field experiment was conducted at Agricultural College and Research Institute, Killikulam during rabi season (October 2018 - January 2019) to evolve suitable little millet (Panicum sumatrense L) based intercropping system under rainfed condition. The experiment was laid out in randomized block design with eight treatments and replicated thrice. The population of soil bacteria, fungi, actinobacteria, Azospirillum and dehydrogenase enzyme activities were recorded under different little millet based intercropping system as a measure of soil microbial dynamics.
12p
chauchaungayxua3
07-02-2020
12
0
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The use of a mixture of both enzyme systems was more effective than using them separately. Under optimal conditions, an increase in oil yield of 41.7 % was achieved, while distillation time was shortened from 8 to 5 hours. The enzyme treatments did not change the qualitative composition of the essential oils. However, significant changes in the percentages of cinnamic aldehyde (69.74 % to 85.6 %) and cinnamyl acetate (17.2 % to 1.34 %) were observed.
7p
cumeo3000
01-08-2018
40
0
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Unlike in dry milling, where the entire mash is fermented, in wet milling only the starch is fermented. The starch is then cooked, or liquefied, and an enzyme added to hydrolyze, or segment, the long starch chains. In dry milling, the mash, which still contains all the feed coproducts, is cooked and an enzyme added. In both systems a second enzyme is added to turn the starch into a simple sugar, glucose, in a process called saccharification. Saccharification in a wet mill may take up to 48 hours, though it usually requires less time, depending on the amount of enzyme used.
222p
loixinloi
08-05-2013
56
9
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We previously reported that GTS1 is involved in regulating ultradian oscillations of the glycolytic pathway induced by cyanide in cell suspensions as well as oscillations of energy metabolism in aerobic continuous cultures. Here, we screened a yeast cDNA library for proteins that bind to Gts1p using the yeast two-hybrid system and cloned multiple TDH cDNAs encoding the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH).
10p
research12
01-06-2013
39
2
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In this Agrodok, we mainly refer to the extensive and semi-intensive farming. Intensive farming with e.g. battery cages will not be treated. When chickens are free to roam and scavenge, we talk about exten- sive, free-range chicken farms. The level of capital and labour invest- ment is low. Housing is not important. Intensive systems, developed for specialised breeds, are estimated to be in use for about 30% of the poultry population in Africa. These are mainly found in and around urban areas with good markets for eggs and chicken meat.
12p
loginnhanh
22-04-2013
51
3
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The localization of different classes of alcohol dehydro-genases (ADH) in the brain is of great interest because of their role in both ethanol and retinoic acid metabolism. Conflicting data have been reported in the literature. By Northern blot and enzyme activity analyses only class III ADH has been detected in adult brain specimens, while results from riboprobe in situ hybridization indicate class I as well as class IV ADH expression in different regions of the rat brain.
11p
tumor12
20-04-2013
38
2
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A search has been initiated for lead inhibitors of the non-structural protein 3 (NS3)-associated NTPase/helicase activities of hepatitis C virus,the related West Nile virus, Japanese encephalitis virus and the human mitochondrial Suv3 enzyme. Random screening of a broad range of unre-lated low-molecular mass compounds,employing both RNA and DNA substrates, revealed that 4,5,6,7-tetra-bromobenzotriazole (TBBT) hitherto known as a potent highly selective inhibitor of protein kinase 2,is a good inhibitor of the helicase,but not NTPase,activity of hepa-titis C virus NTPase/helicase. ...
9p
fptmusic
16-04-2013
38
2
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The mono(ADP-ribosyl)ation reaction is a post-translational modification that is catalysed by both bacterial toxins and eukaryotic enzymes, and that results in the transfer of ADP-ribose from bNAD + to various acceptor proteins. In mammals, both intracellular and extracellular reactions have been described; the latter are due to glycosylphosphatidylinositol-anchored or secreted enzymes that are able to modify their targets, which include the purinergic receptor P2X7, the defensins and the integrins. ...
11p
fptmusic
12-04-2013
34
2
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NAD(P)H regeneration is important for biocatalytic reactions that require these costly cofactors. A mutant phosphite dehydrogenase (PTDH-E175A⁄A176R) that utilizes both NAD and NADP efficiently is a very promising system for NAD(P)H regeneration. In this work, both the kin-etic mechanism and practical application of PTDH-E175A⁄A176R were investigated for better understanding of the enzyme and to provide a basis for future optimization.
12p
fptmusic
11-04-2013
29
1
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The glyoxalase pathway ofLeishmania infantumwas kinetically character-ized as a trypanothione-dependent system. Using time course analysis based on parameter fitting with a genetic algorithm, kinetic parameters were estimated for both enzymes, with trypanothione derived substrates. A Kmof 0.253 mmand aVof 0.21lmolÆmin )1 Æmg )1 for glyoxalase I, and a Kmof 0.098 mmand aVof 0.18lmolÆmin )1 Æmg )1 for glyoxalase II, were obtained.
11p
awards
06-04-2013
44
1
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Planarians are one of the simplest animal groups with a central nervous system. Their primitive central nervous system produces large quantities of a variety of neuropeptides, of which many are amidated at their C terminus. In vertebrates, peptide amidation is catalyzed by two enzymes [peptidylglycine a-hydroxylating monooxygenase (PHM) and peptidyl-a-hydroxylglycinea-amidating lyase] acting sequentially. In mammals, both enzymatic activities are contained within a single protein that is encoded by a single gene....
14p
awards
05-04-2013
39
3
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We report here thatEscherichia coli pyrophosphatase aggregates in the presence of millimolar Cd 2+ . This highly cooperative process was specific to both the metal ion and the protein and could be reversed fully by decreasing the Cd 2+ concentration. Aggregation was enhanced by Mg 2+ , the natural cofactor of pyrophosphatase, and Mn 2+ . Mutations at the intersubunit metal-binding site had no effect, whereas mutation at Glu139, which is part of the peripheral metal-binding site found in pyrophosphatase crystals near the contact region between two enzyme mole-cules, suppressed aggregation.
0p
awards
05-04-2013
32
1
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The creatine⁄creatine kinase system decreases drastically in sarcoma. In the present study, an investigation of catalytic activities, western blot and mRNA expression unambiguously demonstrates the prominent expression of the creatine-synthesizing enzymesl-arginine:glycine amidinotransferase andN-guanidinoacetate methyltransferase in sarcoma, Ehrlich ascites carci-noma and Sarcoma 180 cells, whereas both enzymes were virtually unde-tectable in normal muscle.
11p
vinaphone15
28-02-2013
33
1
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Nitric oxide synthases belong to a family of dual-flavin enzymes that trans-fer electrons from NAD(P)H to a variety of heme protein acceptors. Dur-ing catalysis, their FMN subdomain plays a central role by acting as both an electron acceptor (receiving electrons from FAD) and an electron donor, and is thought to undergo large conformational movements and engage in two distinct protein–protein interactions in the process.
16p
vinaphone15
25-02-2013
27
2
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Rheumatoid arthritis (RA) is a chronic, systemic inflammatory disease primarily of the joints that, if lelt untreated, results in functional disebility concordant with radiographic progression. It is well recognized that strking pathogenic similarities exist between periodontitis and RA. Proinflammatory cytokines such as tumor necrosis factoralpha (TNF a) and interleukin (IL) as well as microbial enzymes and host matrix metalloproteianses (MMP-8 and -9) appear to play important roles in both conditions....
11p
mongmo_tc12c
28-10-2010
106
4
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