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Cartilage regeneration
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Part 2 book "Biomedical tissue culture" includes content: Placental structure and biological aspects of fetal membranes cultured in vitro; tissue development and mechanical property in the regenerated cartilage tissue; the art of animal cell culture for virus isolation; tissue based model of HCV replication as a replacement for animal models in drug testing; tissue culture to assess bacterial enteropathogenicity; viral DNA and CDNA array in the diagnosis of respiratory tract infections.
124p
muasambanhan07
20-02-2024
2
0
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Mesenchymal stem cells are currently a research focus because of the possibility of cartilage regeneration through several mechanisms, including mesenchymal stem cell sheets. However, there are no published reports visualizing cartilage in three dimensions. Here, we report a case of improved cartilage volume.
5p
vilazada
31-01-2024
3
2
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Medical interventions for subchondral bone cysts in horses have been extensively studied. This study investigated the regeneration of articular cartilage and subchondral bone with scaffold-free three-dimensional (3D) constructs of equine synovial membrane-derived mesenchymal stem cells (SM-MSCs) isolated from three ponies and expanded until over 1.0× 107 cells at passage 2 (P2).
16p
vidarwin
22-02-2022
17
1
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The limited capability of articular cartilage to self-repair renders its regeneration a formidable challenge. In the quest of the optimal material for cartilage scaffold fabrication, the use of collagen type II has been advocated, but its optimal source still remains elusive.
9p
meyerowitz
25-12-2021
29
0
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The membrane maintained stability under enzymatic conditions for up to 16 days of incubation. The results confirmed tBP as a cell-friendly scaffold for hADSCs due to low cytotoxicity and its ability to support an appropriate attachment and proliferation of hADSCs. Moreover, there was an accumulation of the extracellular matrix proteoglycan in tBP seeded with hADSCs after 7 and 14 days of chondrogenic induction. COMP as a specific marker of chondrogenesis was detected after 7 days, whereas type X-a1 collagen (Col10a1) expression was stable up to day 14.
11p
thiencuuchu
27-11-2021
10
1
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Injuries to the human native cartilage tissue are particularly problematic because cartilage has little to no ability to heal or regenerate itself. Employing a tissue engineering strategy that combines suitable cell sources and biomimetic hydrogels could be a promising alternative to achieve cartilage regeneration.
10p
vioregon2711
22-02-2021
7
2
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Cartilage tissue engineering is a promising approach for repairing and regenerating cartilage tissue. To date, attempts have been made to construct zonal cartilage that mimics the cartilaginous matrix in different zones.
10p
vioregon2711
22-02-2021
9
2
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There has been increased interest in one-step cell-free procedures to avoid the problems related to cell manipulation and its inherent disadvantages. We have studied the chondrogenic induction ability of a PAMPS/ PDMAAm double-network (DN) gel and found it to induce chondrogenesis in animal osteochondral defect models.
9p
vitennessee2711
02-02-2021
6
3
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It is unclear whether postoperative outcomes are associated with the cartilage regeneration after open wedge high tibial osteotomy (OWHTO) combined with microfracture. The purpose of this study was to evaluate the regeneration of the articular cartilage, radiologic, and clinical outcomes after OWHTO with and without microfracture.
11p
vivermont2711
28-01-2021
6
1
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Synovial mesenchymal stem cells (MSCs) are an attractive cell source for cartilage and meniscus regeneration. The optimum cryopreservation medium has not been determined, but dimethylsulfoxide (DMSO) should be excluded, if possible, because of its toxicity.
9p
vivermont2711
28-01-2021
11
2
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Previous basic research and clinical studies examined the effects of mesenchymal stem cells (MSCs) on regeneration and maintenance of articular cartilage. However, our pilot study suggested that MSCs are more effective at suppressing inflammation and pain rather than promoting cartilage regeneration in osteoarthritis.
11p
vivermont2711
28-01-2021
10
2
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While multiple in vitro studies examined mesenchymal stromal cells (MSCs) derived from bone marrow or hyaline cartilage, there is little to no data about the presence of MSCs in the joint capsule or the ligamentum capitis femoris (LCF) of the hip joint.
13p
vimariana2711
22-12-2020
12
0
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Microfracture is a surgical technique that involves creating multiple holes of 3–4 mm depth in the subchondral bone to recruit stem cells in the bone marrow to the lesion, inducing fibrocartilage repair and knee cartilage regeneration.
7p
vimariana2711
22-12-2020
13
2
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We aimed to evaluate whether arthroscopic microfracture with atelocollagen augmentation could improve the clinical outcomes and quality of regenerated cartilage in patients with osteochondral lesion of the talus (OLT).
12p
vimariana2711
22-12-2020
14
1
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Sonic hedgehog (Shh) is an important signalling protein involved in the induction of early cartilaginous differentiation. Herein, we demonstrate that Shh markedly induces chondrogenesis of rabbit bone marrow stromal cells (BMSCs) under microgravity conditions, and promotes cartilage regeneration.
12p
viwyoming2711
16-12-2020
13
0
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The concept of in situ 3D bio-printing was previously reported, while its realization has still encountered with several difficulties. The present study aimed to report robotic-assisted in situ 3D bio-printing technology for cartilage regeneration, and explore its potential in clinical application. A six-degree-offreedom (6-DOF) robot was introduced in this study, and a fast tool center point (TCP) calibration method was developed to improve printing accuracy. The bio-ink consisted of hyaluronic acid methacrylate and acrylate-terminated 4-armed polyethylene glycol was employed as well.
10p
partimesinhvien
08-05-2020
33
2
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Reactive oxygen species (ROS) are produced as by-products of several intracellular metabolic pathways and are reduced to more stable molecules by several protective pathways. The presence of high levels of ROS can be associated with disturbance of cell function and could lead to apoptosis. The presence of ROS within the physiological range has many effects on several signalling pathways. In stem cells, this role can range between keeping the potency of the naive stem cells to differentiation towards a certain lineage.
7p
trinhthamhodang1
14-11-2019
15
0
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Our study compared the effects of extracorporeal shockwave therapy (ESWT) on the subchondral bone and the articular cartilage in the treatment of early osteoarthritis (OA) of rat knee.
11p
vinasaki2711
12-11-2019
17
2
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Regeneration and healing of bone and cartilage in type-1 and type-2 diabetes: the effects of insulin
Increased fragility of long bones and delay in fracture healing in adults as well as skeletal development disorders in children are thought to be associated with endochondral ossification problems. The extracellular matrix (ECM) of cartilage is absorbed during endochondral ossification.
6p
sony2711
28-01-2019
25
1
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This book describes many aspects of tissue culture models in an extensive manner.
248p
camchuong_1
04-12-2012
95
18
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