CHO cell line
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Human epidermal growth factor receptor 2 (HER2) has been identified as a molecular target for breast cancer therapy, such as Trastuzumab (Herceptin®). This has been shown to improve patient survival substantially.
10p vibego 02-02-2024 0 0 Download
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In the present study, attempts were made to produce biologically active recombinant mouse hephaestin as a secretory form tagged with green fluorescent protein (GFP), Hpsec-GFP. Plasmid expressing Hpsec-GFP was constructed and transfected into COS and CHO cells. The GFP aided the monitoring expression in real time to select the best conditions to maximise expression and provided a tag for purifying and analysing Hpsec-GFP. The protein had detectable oxidase activity as shown by in-gel and solution-based assays.
13p thiencuuchu 27-11-2021 8 1 Download
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Tế bào gốc là tế bào có khả năng hóa thành các tế bào khác nhau để thay thế cho tế bào già và chết tự nhiên hay do chấn thương vì nhiều nguyên nhân khác nhau, mở ra triển vọng sử dụng TBG to tái tạo lại các mô. Ghép TBG từ tuỷ xương tự thân đã được sử dụng trên lâm sàng để điều trị thành công các trường cắt đứt liên kết lâu dài và giả mạo. Khi được tiêm vào xương gãy, xương TBG sẽ hóa thành tế bào dạng xương (TBDTX), tái tạo lại ố khuyết xương. Mời các bạn cùng tìm hiểu nội dung bài báo.
8p closefriend02 07-10-2021 28 2 Download
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Chinese hamster ovary (CHO) cells have become the host of choice for the production of recombinant proteins, due to their capacity for correct protein folding, assembly, and posttranslational modifications.
10p viuchiha2711 21-04-2020 10 0 Download
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Recombinant cell lines developed for therapeutic antibody production often suffer instability or lose recombinant protein expression during long-term culture. Heterogeneous gene expression among cell line subclones may result from epigenetic modifications of DNA or histones, the protein component of chromatin.
16p viuchiha2711 21-04-2020 14 0 Download
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As the demand for monoclonal antibodies (mAb) increases, more efficient expression methods are required for their manufacturing process. Transcriptional gene silencing is a common phenomenon in recombinant cell lines which leads to expression reduction and instability.
9p vihamax2711 21-04-2020 10 2 Download
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Recombinant factor VIII (FVIII), used for haemophilia A therapy, is one of the most challenging among the therapeutic proteins produced in heterologous expression systems. Deletion variant of FVIII, in which the entire domain B is replaced by a short linker peptide, was approved for medical use.
15p vihamax2711 21-04-2020 17 2 Download
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Monoclonal antibodies (mAbs) are crucial in pharmaceutical biotechnology. Mammalian cell lines are the most preferred for their production. One of the significant challenging issues of mammalian expression systems is epigenetic gene silencing.
7p nutifooddau 27-01-2019 17 1 Download
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As human choriocarcinoma cells display many of the biochemical and morphological characteristics reported for in utero invasive trophoblast cells we have studied cho-lesterol supply from high density lipoproteins (HDL) to these cells.Binding properties of 125 I-labeled HDL sub-class 3 (HDL3)at4C were similar for BeWo, JAr, and Jeg3 choriocarcinoma cell lines while degradation rates at 37C were highest for BeWo.
12p tumor12 20-04-2013 31 1 Download
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The conduction of protons through human Nox2 has pre-viously been shown to be dependent upon His115. Align-ment of sequences for both animal and plant Nox proteins indicated that histidines 115 and 119 are both highly con-served, while His111 was conserved among animal homo-logues of Nox1–4. To investigate the possible role that these histidine residues might play in the conduction of protons through Nox2, we have introduced both paired and single mutations into these histidine residues.
8p awards 05-04-2013 41 3 Download
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Glycoprotein (GP) V is noncovalently linked to GPIba, GPIbband GPIX within the platelet GPIb–V–IX complex, a receptor for von Willebrand factor and thrombin. Two functions have been ascribed to GPV, namely, the modula-tionof thrombin- andcollagen-dependent platelet responses. The biosynthesis of this molecule was investigated in pulse– chase metabolic labelling experiments performed in CHO cell lines transfected with GPV, alone or in the presence of GPIb–IX.
0p awards 05-04-2013 29 1 Download
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The effects of protection of chylomicron remnants from oxidation on their uptake and induction of lipid accumula-tion in macrophages were investigated using chylomicron remnant-like particles (CRLPs) containing the lipophilic antioxidant drug, probucol, andmacrophages derived from thehumanmonocyte cell line,THP-1.The total lipidcontent of THP-1 macrophages was markedly higher (·2.2) after 48 h of incubation of THP-1 macrophages with CRLPs containing probucol (pCRLPs) when compared to CRLPs without probucol, and this was because of increases in tri-acylglycerol (·2.3) and cholesterol (·1.
11p dell39 03-04-2013 55 3 Download
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Endoplasmic reticulum-associated degradation of newly synthesized glycoproteins has been demonstrated previously using various mammalian cell lines. Depending on the cell type, glycoproteins bearingMan9 glycans and glycoproteins bearing Man5 glycans can be efficiently degraded. Awide variety of variables can lead to defective synthesis of lipid-linked oligosaccharides and, therefore, in mammalian cells, species derived fromMan9GlcNAc2 orMan5GlcNAc2 are often recovered on newly synthesized glycoproteins. ...
7p dell39 03-04-2013 30 2 Download
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Các thực thể y sinh phổ biến: DNA, RNA, Cell line, Cell type, Gene… Disease (bệnh), Symptom (triệu chứng), Virus, Atom… = Kiểu hình(Phenotype), Gene, Bệnh (Disease) , Hóa chất (Chemical) Protein,
23p bluesky_12 21-12-2012 80 9 Download
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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Comparison of In vitro Nanoparticles Uptake in Various Cell Lines and In vivo Pulmonary Cellular Transport in Intratracheally Dosed Rat Model
9p sting09 21-02-2012 41 4 Download
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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Quantitative profiling of housekeeping and Epstein-Barr virus gene transcription in Burkitt lymphoma cell lines using an oligonucleotide microarray
15p dauphong7 13-01-2012 49 2 Download
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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Inhibition of histone deacetylation in 293GPG packaging cell line improves the production of self-inactivating MLV-derived retroviral vectors
12p dauphong7 13-01-2012 43 2 Download
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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Respiratory syncytial virus glycoproteins uptake occurs through clathrin-mediated endocytosis in a human epithelial cell line
4p dauphong7 13-01-2012 37 2 Download
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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Expression of frog virus 3 genes is impaired in mammalian cell lines
7p dauphong7 13-01-2012 46 2 Download
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Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành hóa học dành cho các bạn yêu hóa học tham khảo đề tài: Dig1 protects against cell death provoked by glyphosate-based herbicides in human liver cell lines
13p panasonic09 13-01-2012 33 2 Download