Chymotrypsin

Bài viết Ảnh hưởng của nhiệt độ kết hợp độ mặn lên sinh lý, tăng trưởng và men tiêu hóa của cá lóc (Channa striata) giai đoạn cá bột lên cá giống được nghiên cứu nhằm đánh giá ảnh hưởng kết hợp của nhiệt độ và độ mặn lên sinh lý, tăng trưởng và hoạt tính enzyme tiêu hoá của cá lóc (Channa striata) giai đoạn cá bột lên cá giống.

11p vimelindagates 18-07-2022 23 5   Download

Nghiên cứu này được thực hiện nhằm mục đích tìm ra phương pháp hiệu quả để thu nhận chế phẩm protease từ tụy tạng lợn và ứng dụng vào việc làm mềm thịt. Kết quả cho thấy phương pháp chiết enzyme bằng nước cất rồi kết tủa protein enzyme bằng ethanol 96% đã cho hiệu quả thu nhận chế phẩm enzyme cao. Chế phẩm này có mặt các enzyme trypsin, chymotrypsin, pepsin và có khả năng làm mềm thịt ở hàm lượng 0,5% và có tỷ lệ mất nước trong chế biến không đáng kể.

7p viwendy2711 05-10-2021 30 3   Download

Bài viết tiến hành nghiên cứu xây dựng và thẩm định quy trình định lượng trypsin trong viên nén bao tan trong ruột trypsin – chymotrypsin nhằm đưa ra phương pháp có thể dùng để định lượng trypsin trong viên nén kết hợp 2 enzym trypsin và chymotrypsin.

5p nguathienthan12 22-05-2021 58 1   Download

The metabolic activities of the casein peptides were verified using purified enzymatic inhibition studies. The regulatory enzyme inhibition led us to conclude a positive correlation with the role of casein peptides in modulating the diabetic and hypertensive metabolic pathways in the biological system. Purified enzymes were used for the investigation to clearly observe the direct impact on the diabetes and hypertension related bioprocesses.

10p nguaconbaynhay7 15-08-2020 15 1   Download

Department of Biochemistry, Eotvos Lorand University, and 2Biotechnology Research Group of the Hungarian Academy of Sciences, ´ ´ ´ ´ Pazmany setany 1/C, Budapest, Hungary The molecular mechanism of the autolysis of rat a-chymotrypsin B was investigated. In addition to the two already known autolytic sites, Tyr146 and Asn147, a new site formed by Phe114 was identified. The former two sites and the latter one are located in the autolysis and the interdomain loops, respectivel

9p system191 01-06-2013 36 4   Download

Duodenase is a 29-kDa serine endopeptidase that displays selective trypsin- and chymotrypsin-like substrate specificity. This enzyme has been localized to epitheliocytes of Brunner’s glands, and as described here, to mast cells within the intestinal mucosa and lungworm-infected lung, implying an important additional role in inflammation and tissue remodelling. In primary cultures of pulmonary artery fibroblasts, duodenase induced a concentration-dependent increase in [3H]thymidine incorporation with a maximal effect observed at 30 nM.

10p research12 01-06-2013 52 3   Download

Bovineb-lactoglobulin was hydrolyzed with trypsin or chymotrypsin in the course of heat treatment at 55, 60 and 65°C at neutral pH. At these temperaturesb-lactoglobulin undergoes signi®cant but reversible structural changes. In the conditions used in the present study,b-lactoglobulinwas virtually insensitive to proteolysis by either enzyme at room temperature, but underwent extensive proteolysis when either proteasewas present during the heat treatment.

11p research12 29-04-2013 33 3   Download

Achromobacterprotease I (API) has a unique region of aromatic ring stacking with Trp169–His210in close proxi-mity to the catalytic triad. This paper reveals the electrostatic role of aromatic stacking in the shift in optimum pH to the alkaline region, which is the highest pH range (8.5–10) among chymotrypsin-type serine proteases. The pH-activity profile of API showed a sigmoidal distribution that appears at pH 8–10, with a shoulder at pH 6–8.

7p research12 23-04-2013 41 2   Download

The solution structure of three small serine proteinase inhibitors, two natural and one engineered protein, SGCI (Schistocerca gregaria chymotrypsin inhibitor), SGCI[L30R, K31M] and SGTI (Schistocerca gregaria trypsin inhibitor), were determined by homonuclear NMR-spectroscopy. The molecules exhibit di€erent speci®cities towards target proteinases, where SGCI is a good chymo-trypsin inhibitor, itsmutant is apotent trypsin inhibitor, and SGTI inhibits both proteinases weakly.

11p research12 23-04-2013 33 2   Download

Although thea-chymasesof primates anddogs areknownas chymotrypsin-like proteases, the enzymatic properties of rodent a-chymases (rat mast cell protease 5/rMCP-5 and mouse mast cell protease 5/mMCP-5) have not been fully understood. We report that recombinant rMCP-5 and mMCP-5 are elastase-like proteases, not chymotrypsin-like proteases. An enzyme assay using chromogenic peptidyl substrates showed that mast cell protease-5s (MCP-5s) have a clear preference for small aliphatic amino acids (e.g. alanine, isoleucine, valine) in the P1 site of substrates....

10p tumor12 22-04-2013 36 2   Download

Butyrylcholinesterase is a serine esterase, closely related to acetylcholinesterase. Both enzymes employ a catalytic triad mechanism for catalysis, similar to that used by serine pro-teases such as a-chymotrypsin. Enzymes of this type are generally considered to be inactive at pH values below 5, because the histidine member of the catalytic triad becomes protonated. We have found that butyrylcholinesterase retains activity at pH £ 5, under conditions of excess substrate activation.

10p tumor12 20-04-2013 25 4   Download

Chymotrypsin is a prominent member of the family of serine proteases. The present studies demonstrate the presence of a native fragment contain-ing 14 residues from Ile16 to Trp29 in a-chymotrypsin that binds to chy-motrypsin at the active site with an exceptionally high affinity of 2.7 ± 0.3·10 )11 mand thus works as a highly potent competitive inhib-itor. The commercially available a-chymotrypsin was processed through a three phase partitioning system (TPP). The treated enzyme showed consid-erably enhanced activity. ...

11p awards 05-04-2013 48 3   Download

Complexation of the small serine protease inhibitorSchistocerca gregaria chymotrypsin inhibitor (SGCI), a member of the pacifastin inhibitor family, with bovine chymotrypsin was followed by NMR spectroscopy. 1 H– 15 N correlation (HSQC) spectra of the inhibitor with increasing amounts of the enzyme reveal tight and specific binding in agreement with biochemical data.

12p inspiron33 26-03-2013 47 6   Download

The 3C-like protease of the severe acute respiratory syndrome (SARS) cor-onavirus has a C-terminal extra domain in addition to the chymotrypsin-fold adopted by piconavirus 3C proteases hosting the complete catalytic machinery. Previously we identified the extra domain to be involved in enzyme dimerization which has been considered essential for the catalytic activity.

11p inspiron33 26-03-2013 40 4   Download

Although the chemical nature of the catalytic mechanism of the serine pro-tease a-chymotrypsin (a-CT) is largely understood, the influence of the enzyme’s structural dynamics on its catalysis remains uncertain. Here we investigate whether a-CT’s structural dynamics directly influence the kinet-ics of enzyme catalysis.

17p inspiron33 23-03-2013 31 3   Download

The yeast 20S proteasome is subject to sulfhydryl redox alterations, such as the oxidation of cysteine residues (Cys-SH) into cysteine sulfenic acid (Cys-SOH), followed by S-glutathionylation (Cys-S-SG). Proteasome S-glutath-ionylation promotes partial loss of chymotrypsin-like activity and post-acidic cleavage without alteration of the trypsin-like proteasomal activity.

14p galaxyss3 07-03-2013 36 5   Download

Chymases are chymotrypsin-like serine proteases that are found in large amounts in mast cell granules. So far, the extended cleavage specificities of eight such chymases have been determined, and four of these were shown to have a strong preference for acidic amino acids at position P2¢. These enzymes have basic amino acids in positions 143 and 192 (Arg and Lys, respectively).

13p mobifone23 21-01-2013 46 5   Download

Cathepsin G (CG) (EC 3.4.21.20) and chymase (EC 3.4.21.39) are two clo-sely-related chymotrypsin-like proteases that are released from cytoplasmic granules of activated mast cells and⁄or neutrophils. We investigated the potential for their substrate-binding subsites to discriminate between their substrate specificities, aiming to better understand their respective role dur-ing the progression of inflammatory diseases.

12p cosis54 04-01-2013 31 4   Download

Công trình này nghiên cứu tác dụng của dịch chiết từ các phần khác nhau của thân, hạt cây tô mộc (C.sappan) đến hoạt độ của một số proteinase. Các kết quả nghiên cứu cho thấy: Dịch chiết từ phần vỏ cũng như phần gỗ thân cây C. sappan đều có hoạt tính ức chế trypsin (TIA) và hoạt tính ức chế chymotrypsin (ChIA). TIA , ChIA của phần gỗ và lõi gỗ theo thứ tự vào khoảng 6,11 IU và 22,44 IU/100 gam nguyên liệu ; TIA , ChIA của phần vỏ thân theo thứ tự vào khoảng 52,45 IU...

10p tuanlocmuido 13-12-2012 73 11   Download

Human chymotrypsin C (CTRC) plays a protective role in the pancreas by mitigating premature trypsinogen activation through degradation. Mutations that abolish activity or secretion of CTRC increase the risk for chronic pancreatitis.

13p cosis54 09-12-2012 40 2   Download