Coa gene

Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most predominant agents that cause nosocomial infections. Objectives: To determine the rate of MRSA and the molecular characteristics of the coagulase encoding gene of these isolates based on polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP).

6p viuchiha 03-01-2025 2 2   Download

4-Coumarate:CoA ligase (4CL) is involved in the formation of coenzyme A thioesters of hydroxycinnamic acids that are central substrates for subsequent condensation, reduction, and transfer reactions in the biosynthesis of plant phenylpropanoids. Previous studies of 4CL appear to suggest that many isoenzymes are functionally equivalent in supplying substrates to various subsequent branches of phenylpropanoid biosyntheses. In contrast, divergent members of a 4CL gene family were identified in soybean (Glycine max L.).

12p research12 01-06-2013 55 5   Download

Propionate CoA-transferase fromClostridium propionicum hasbeenpuri®edand thegene encoding the enzymehasbeen cloned and sequenced. The enzyme was rapidly and irre-versibly inactivated by sodium borohydride or hydroxyl-amine in the presence of propionyl-CoA. The reduction of the thiol ester between a catalytic site glutamate and CoA with borohydride and the cleavage by hydroxylamine were used to introduce a site-speci®c label, which was followed by MALDI-TOF-MS. This allowed the identi®cation of glutamate 324 at the active site. ...

9p research12 29-04-2013 32 4   Download

In rodents, the olfactory epithelium(OE) canbe divided into four topographically distinct zones, and eachmember of the odorant receptor(OR) gene family is expressed only in one particular zone. To study the functional significance of the zonal structure of the OE, we searched for genes expressed in a zone-specific manner by using the differential display method. Among the clones isolated from the rat OE, we characterized a novel olfactory protein termed O-MACS, a member of the medium-chain acyl-CoA synthetase family....

10p fptmusic 16-04-2013 40 3   Download

3-Methyl-branched fatty acids, as phytanic acid, undergo peroxisomala-oxidation in which they are shortened by 1 carbon atom. This process includes four steps: activation, 2-hydroxylation, thiamine pyrophosphate dependent cleavage and aldehyde dehydrogenation. The thiamine pyrophosphate dependence of the third step is unique in peroxisomal mammalian enzymology. Human pathology due to a deficient alpha-oxidation is mostly linked to mutations in the gene coding for the second enzyme of the sequence, phytanoyl-CoA hydroxylase....

9p fptmusic 16-04-2013 41 2   Download

Two novel rare mutations, MCAD
842GfiC (R256T) and MCAD
1166AfiG (K364R), have been investigated to assess how far the bio-chemical properties of the mutant proteins correlate with the clinical phenotype of medium chain acyl-CoA dehydrogenase (MCAD) deficiency. When the gene for K364R was overexpressed inEscherichia coli, the syn-thesized mutant protein only exhibited activity when the gene for chapero-nin GroELS was co-overexpressed.

9p fptmusic 12-04-2013 45 2   Download

Apple flavor is characterized by combinations of ester compounds, which increase markedly during fruit ripening. The final step in ester biosynthesis is catalyzed by alcohol acyl transferases (AATs) that use coenzyme A (CoA) donors together with alcohol acceptors as substrates. The gene MpAAT1, which produces a predicted protein containing features of other plant acyl transferases, was isolated fromMalus pumila(cv. Royal Gala). TheMpAAT1gene is expressed in leaves, flowers and fruit of apple.

13p fptmusic 11-04-2013 41 2   Download

Methylmalonyl-CoA epimerase (MCE) is an enzyme involved in the pro-pionyl-CoA metabolism that is responsible for the degradation of branched amino acids and odd-chain fatty acids. This pathway typically functions in the reversible conversion of propionyl-CoA to succinyl-CoA. The Caenor-habditis elegansgenome contains a single gene encoding MCE (mce-1) cor-responding to a 15 kDa protein.

13p awards 06-04-2013 39 3   Download

ThehadBC and hadI genes from Clostridium difficilewere functionally expressed inEscherichia coliand shown to encode the novel 2-hydroxyiso-caproyl-CoA dehydratase HadBC and its activator HadI. The activated enzyme catalyses the dehydration of (R)-2-hydroxyisocaproyl-CoA to isocaprenoyl-CoA in the pathway of leucine fermentation. The extremely oxygen-sensitive homodimeric activator as well as the heterodimeric dehy-dratase, contain iron and inorganic sulfur; besides varying amounts of zinc, other metal ions, particularly molybdenum, were not detected in the dehy-dratase....

12p awards 05-04-2013 31 2   Download

Malonyl-CoA, a potent inhibitor of carnitine pamitoyl transferase-I (CPT-I), plays a pivotal role in fuel selection in cardiac muscle. Malonyl-CoA decarboxylase (MCD) cata-lyzes the degradation of malonyl-CoA, removes a potent allosteric inhibition on CPT-I and thereby increases fatty acid oxidation in the heart. Although MCDhas several Ser/ Thr phosphorylation sites, whether it is regulated by AMP-activated protein kinase (AMPK) has been controversial. We therefore overexpressed MCD(Ad.MCD) and consti-tutively active AMPK (Ad.

10p dell39 03-04-2013 39 3   Download

Mitochondrial fatty acid oxidation deficiencies are due to genetic defects in enzymes of fatty acidb-oxidation and transport proteins.Genetic defects have been identified in most of the genes where nearly all types of sequence vari-ations (mutation types) have beenassociatedwithdisease.In this paper, we will discuss the effects of the various types of sequence variations encountered and review current know-ledge regarding the genotype–phenotype relationship, espe-cially in patients with acyl-CoA dehydrogenase deficiencies where sufficient material exists for a meaningful discussion....

13p dell39 03-04-2013 49 3   Download

The expression of the colonic mitochondrial 3-hydroxy 3-methyl glutaryl CoA (mHMGCoA) synthase,a key con-trol site of ketogenesis from butyrate,is lower in germ-free (GF) than in conventional (CV) rats. In contrast,the activity of glutaminase is higher. The objective of this study was to investigate whether the intestinal flora can affect gene expression through short chain fatty acid (SCFA) and butyrate production. GF rats were inoculated with a con-ventional flora (Ino-CV) orwitha bacterial strainproducing butyrate (Clostridium paraputrificum,Ino-Cp) or not (Bifidobacterium breve,Ino-Bb).

9p dell39 03-04-2013 29 4   Download

The linear biosynthetic pathway leading froma-ketoisovalerate to panto-thenate (vitamin B5) and on to CoA comprises eight steps in the Bacteria and Eukaryota. Genes for up to six steps of this pathway can be identified by sequence homology in individual archaeal genomes.

11p media19 06-03-2013 35 3   Download

The isovaleric acid-emanating silkworm mutantskunk (sku) was first stud-ied over 30 years ago because of its unusual odour and prepupal lethality. Here, we report the identification and characterization of the gene responsi-ble for theskumutant.

12p viettel02 19-02-2013 33 2   Download