Combined disc test

Antimicrobial resistance is a growing threat worldwide. Increasing resistance to third generation cephalosporins has become a cause for concern. The prevalence of extended spectrum–lactamases (ESBLs) and metallobetalactamases (MBL) among members of Enterobacteriaceae constitutes a serious threat to current -lactam therapy leading to treatment failure. A total of 276 Gram negative isolates were processed. ESBL was detected by phenotypic confirmatory disc diffusion test (PCDDT) using ceftazidime alone and in combination with clavulanic acid.

8p angicungduoc6 20-07-2020 19 1   Download

Acinetobacter the aerobic non fermenting GNCB are emerging as important pathogens in health care associated infection, exhibiting increased antimicrobial resistance by virtue of multiple resistant mechanisms with practically no therapeutic options. The objective was to determine the multitude of resistant mechanisms by phenotypic and genotypic characterization and the prevalent antimicrobial susceptibility pattern. 175 clinically significant non-duplicate Acinetobacter isolates were included.

10p nguaconbaynhay6 23-06-2020 12 1   Download

The aim of this study was to analyze the extended spectrum of β lactamase (ESBL), metallo β lactamase (MBL) and AmpC production in Pseudomonas aeruginosa in various clinical samples. A Total of 100 clinical isolates of P. aeruginosa were collected from different clinical specimen and confirmed by standard tests. Antibiotic susceptibility was determined by the Kirby-Bauer disc diffusion method. ESBL screening was done using 3rd generation cephalosporins and confirmatory combined double disc test, imipenem-EDTA double disc synergy test for MBL enzyme and AmpC test using Cefoxitin disc.

9p trinhthamhodang1213 30-05-2020 11 1   Download

Non fermenting Gram Negative Bacilli (NFGNB) once considered as contaminants, now emerged as a major cause of life threatening nosocomial infections and as multidrug resistant pathogens. Acinetobacter species are the opportunistic pathogens with increasing prevalance in the nosocomial infections. Community acquired infections are also common in Acinetobacter. It accounts for 10% of all community-acquired bacteremic pneumonias. To isolate, identify and detect Carbapenem resistance producing Acinetobacter spp., and confirm Metallo Beta Lactamase ( MBL) production by phenotypic methods.

10p trinhthamhodang5 16-05-2020 15 1   Download

The present study was done to detect Extended spectrum β lactamase and metallo-βlactamase producing isolates of Pseudomonas aeruginosa from various clinical samples from patients admitted in Tirunelveli Government medical college hospital. In this study we studied the prevalence of ESBL and MBL among 112 Pseudomonas aeruginosa isolates, following standard methods of isolation and identification of these bacteria from clinical materials

8p kethamoi4 18-04-2020 15 0   Download

Resistance to carbapenems is an emerging problem among Klebsiella pneumoniae isolates. A simple and effective phenotypic detection method for carbapenemase production is required in health care settings. A total of 100 clinical isolates of Klebsiella pneumoniae from various samples were screened for imipenem resistance. The imipenem resistant strains were further tested for metallo-beta-lactamase (MBL) production by imipenem combined disc test and ceftazidime combined disc test.

6p kethamoi4 16-04-2020 8 1   Download

A laboratory test-rig for groundnut planter was developed for testing and calibrating the pneumatic seed metering mechanism. The experimental test rig mainly consists of main frame, seed hopper, seed metering system, power transmission system and sticky belt system. The metering unit is incorporated with a vertical seed plate held in a vacuum retaining chamber to singulate the seeds. A dial type vacuum gauge was provided in order to record the variation in suction pressure. A sticky belt was used to simulate the field surface during laboratory investigation.

7p caygaocaolon3 09-03-2020 15 0   Download

Antimicrobial resistance pattern and resistance to third generation cephalosporins has become increased worldwide. The detection of extended spectrum lactamases (ESBLs) and metallobetalactamases (MBLs) among members of Enterobacteriaceae family guide us to use Beta lactam, if not tested leading to treatment failure. One year prospective study, our aim is to detect ESBL and MBL producing gram negative bacilli from various clinical samples. A total of 100 Gram negative bacilli were processed.

7p caygaocaolon3 09-03-2020 20 0   Download

The incidence of infections caused by multidrug resistant ESBL producing K.pneumoniae is on rise. The treatment of these infections have become increasingly difficult and life threatening. Knowledge on prevalence of these infections and corresponding antimicrobial susceptibility patterns is essential regionally. Routine standard operative procedures were followed in the laboratory in processing the clinical specimens for culture and sensitivity. ESBL producing K.pneumoniae were identified by phenotypic combination disc test. Majority of K.

6p cothumenhmong3 22-02-2020 24 2   Download

Metallo beta-lactamase (MBL) and biofilm production is the most worrisome resistant mechanisms observed in Pseudomonas aeruginosa. Increased resistance of Pseudomonas to antibiotic therapy is an emerging global health concern which can be overcomed by Silver nanoparticles (AgNPs). Therefore this study aims to evaluate the antibacterial and antibiofilm activity of Silver nanoparticles on MBL and biofilm producing P. aeruginosa isolates using Transmission Electron microscope (TEM) and Confocal laser scanning microscope (CLSM) respectively. A total 40 isolates of p.

18p chauchaungayxua3 07-02-2020 11 0   Download

MBL producing members of family Enterobacteriaceae have been responsible for numerous outbreaks of infection throughout the world. A total of 108 non repetitive carbapenem resistant strains isolated from various clinical samples, identified by standard microbiological techniques and antimicrobial susceptibility pattern was determined. MBL detection was done by combined disc test using ceftazidime-EDTA, imipenemEDTA and meropenem-EDTA. Ceftazidime-EDTA detected 90 (97.8%) isolates, imipenem-EDTA detected 14 (15.2%) isolates and meropenemEDTA detected 13 (14.

5p nguaconbaynhay3 07-02-2020 24 1   Download

Carbapenemase producing carbapenem resistant Enterobacteriacea were almost non-existent till 1990s but nowadays they are routinely encountered in hospitals. KPC producing Klebsiella pneumoniae were the first to develop and occur commonly. Lately NDM producing Enterobacteriaceae have emerged. IMP and VIM genes in Carbapenem resistant Enterobacteriaecae have started to emerge but the prevalence is low.

7p quenchua2 15-12-2019 8 0   Download

One of the common causes of morbidity and mortality following burn injury is infections. The emergence of metallo-β-lactamase (MBL) in Gram negative bacilli (GNB) has reduced the available therapeutic options for effective treatment. Therefore, for screening of carbapenemase producers, a simple and inexpensive testing method is essential. To know the prevalence of MBL production in various gram negative isolates, to evaluate different phenotypic methods to detect MBL production and to find out antibiotic sensitivity profile of MBL producing gram negative bacilli.

9p quenchua2 15-12-2019 14 0   Download

Carbapenem antibiotics are very often used against multidrug resistant strains clinically troublesome pathogens which developed and proved that the resistance and metallo-βlactamases (MBL) production were a disaster in treating infections. The identification and detection of MBL-producing bacterial strains were having crucial importance for the prevention of nosocomial infections. Therefore the present study was undertaken for screening MBL production Gram Negative bacteria.

8p quenchua2 15-12-2019 13 1   Download

Non-fermenting gram-negative bacilli (NFGNB) are an emerging problem in Blood stream infections. A major concern is multi-drug resistance which severely limits treatment options. Earlier it was believed to be non pathogenic, but recently they are more frequently isolated as primary pathogen. Usually they cause hospital acquired infection (HAI). A prospective study was conducted to isolate the NFGNB from blood samples, to identify the risk factors leading to blood stream infections and to determine the antibiotic susceptibility pattern of them.

14p nguaconbaynhay1 04-12-2019 17 1   Download

ESL students, TOEFL and TOEIC test takers, and all others for whom English is a second language and who want to improve their pronunciation will find this book and compact disc combination especially instructive. The new second edition presents a significantly increased number of exercises, all of them providing practice in every sound that is specific to American-style English. Emphasis is on stress and intonation patterns in conversational English. Recognition of these patterns is essential to fluent speech and listening comprehension.

104p nmtinh 26-11-2010 394 136   Download