Denaturing gradient gel electrophoresis

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Ebook Environmental microbiology - Methods and protocols (2/E): Part 1

Part 1 book "Environmental microbiology - Methods and protocols" includes content: Methods for isolation and cultivation of filamentous fungi, rapid extraction of PCR-competent DNA from recalcitrant environmental samples, quantitative PCR for detection of mRNA and gDNA in environmental isolates, analysis of community dynamics in environmental samples using denaturing gradient gel electrophoresis, terminal restriction fragment length polymorphism (T-RFLP) profiling of bacterial 16S rRNA genes,... and other contents.

134p oursky10 04-12-2023 4 2 Download

Analysis of the bacterial community in chronic obstructive pulmonary disease sputum samples by denaturing gradient gel electrophoresis and real-time PCR

The Global Initiative defines COPD for chronic obstructive lung disease as an entirely preventable and treatable disease characterized by sputum production, bacterial colonisation, neutrophilic bronchial airway inflammation and poor health status.

7p vimontana2711 05-04-2021 9 1 Download

Bacterial community analysis of soybean (Glycine max) Sprayed with panchagavya revealed by DGGE

Present study was aimed to understand the bacterial community composition, diversity, richness, carrying capacity and population dynamics present on soybean leaf in response to Panchagavya sprays using DGGE (Denaturing Gradient Gel Electrophoresis) and molecular fingerprinting technique. The seeds of soybean variety JS-335 were collected, surface sterilized and used in a pot culture study. In-vivo pot experiment was carried out in four sets.

11p trinhthamhodang6 09-07-2020 13 1 Download
Ecotoxicology inside the gut: Impact of heavy metals on the mouse microbiome

The gut microbiota is critical for intestinal homeostasis. Recent studies have revealed the links between different types of dysbiosis and diseases inside and outside the intestine. Environmental exposure to pollutants (such as heavy metals) can also impair various physiological functions for good health.

11p vienzym2711 03-04-2020 9 1 Download
Báo cáo " An active PAH-degrading microbial consortium developed from dioxin-contaminated sediments via enrichment technique "

Via enrichment procedures carried out on a dioxin contaminated sediment sample from lotus pond at Danang airport, a microbial community assigned as DN553 with high capability of carbazol degradation was established. Analyses of community structure by using denaturing gradient gel electrophoresis (DGGE) of 16S rDNA fragments indicated that Achromobacter and Alcaligenes species dominated in this enrichment culture.

7p tuanlocmuido 13-12-2012 34 3 Download