Dependency growth

Epigenetic defects (gain or loss of DNA methylation) of the human ICR1 11p15 domain result in two opposite foetal growth disorders (BWS and SRS) depending on which parental allele is affected. A few deletions within ICR1 have been reported in familial BWS cases with ICR1 gain of methylation, however the mechanism(s) of the DNA methylation defects at ICR1 remains largely unknown in most BWS and SRS patients.

83p runthenight07 01-03-2023 9 3   Download

These results revealed strain-dependent antifungal activity. Our study indicates that the use of these lactic acid bacteria can be applied as a biocontrol of fungal growth and mycotoxins production.

6p mudbound 10-12-2021 6 1   Download

We study the higher differentiability of stationary solutions up to the flat boundary for a class of systems of fluid mechanics modelling flows of incompressible fluids with shear and pressure dependent viscosity in 2D or 3D. We consider systems which are a kind of generalized Navier-Stokes system where stress tensor T has linear growth in symmetric velocity gradient and dependence of viscosity on the pressure is small with respect to an ellipticity constant.

8p tamynhan5 10-12-2020 9 1   Download

In fruit production, the effects of flowering and fruiting, which modify source-sink relationships, cannot be overlooked and is even more true in tropical fruit production, where the flowering and fruiting phases exceed six months at the individual tree scale. More specifically, little is known about the effects of source/sink balance, and the associated changes in carbon export rate from leaves and leaf carbohydrate concentration, like the ones resulting from the presence of developing fruits, on leaf nitrogen and photosynthetic capacity within the crown of field-growing trees.

10p chauchaungayxua6 26-06-2020 12 2   Download

This paper conducts a pilot research on the relationship between industrial policy quality and growth performance. A middle income trap is defined as a situation where the domestic economy is unable to create value beyond what is delivered by given advantages. Given advantages include natural, demographic and geographical factors as well as such external factors as trade, aid, and foreign investment inflow. When growth depends mainly on these factors, little domestic value is created and the economy does not reach high income.

11p truongtien_08 06-04-2018 39 3   Download

The transcription factor serum response factor (SRF) regulates expression of growth factor-dependent genes and muscle-specific genes in vertebrates. Homologous factors regulate differentiation of some ectodermic tissues in invertebrates. To explore the molecular basis of these different physiological functions, the functionality of human, Drosophila melanogaster and Artemia franciscana SRFs in mammalian cells has been compared in this article. D. melanogaster and, to a lesser extend, A.

9p system191 01-06-2013 32 5   Download

An unconventional phospholipase D (PLD) activity was identified recently in Saccharomyces cerevisiae which is Ca2+-dependent, preferentially hydrolyses phosphatidylethanolamine (PtdEtn) and phosphatidylserine and does not catalyse a transphosphatidylation with primary short-chain alcohols. We have characterized the cytosolic and membrane-bound forms of the yeast PtdEtn-PLD and examined the regulation of its activity under certain growth, nutritional and stress conditions. Both forms of PtdEtn-PLD activity were similarly activated by Ca2+ ions in a biphasic manner.

10p system191 01-06-2013 36 4   Download

The interaction of blood coagulation factor X and its Gla-containing fragments with negatively charged phospholipid membranes composed of 25 mol% phosphatidylserine (PtdSer) and 75 mol% phosphatidylcholine (PtdCho) was studied by surface plasmon resonance. The binding to 100 mol% PtdCho membranes was negligible. The calcium dependence in the membrane binding was evaluated for intact bovine factor X (factor X) and the fragment containing the Gla-domain and the N-terminal EGF (epidermal growth factor)-like domain, Gla–EGFN, from factor X....

6p system191 01-06-2013 30 4   Download

The eukaryotic glyoxylate cycle has been previously hypothesized to occur in the peroxisomal compartment, which in the yeast Saccharomyces cerevisiae additionally represents the sole site for fatty acid b-oxidation. The subcellular location of the key glyoxylate-cycle enzyme malate synthase 1 (Mls1p), an SKL-terminated protein, was examined in yeast cells grown on di€erent carbon sources.

8p research12 01-06-2013 42 4   Download

The promoter of the early growth response gene (Egr-1) has been described to be activated by ionizing radiation, and it seems to be clear that this process involves di€erent mitogen activated protein (MAP) kinases, dependent on the speci®c cell type examined. However, early steps leading to activation of the corresponding pathways and thus to overexpression of Egr-1 are not well understood.

10p research12 29-04-2013 45 2   Download

Eosinophil cationic protein (ECP), one of the major components of basic granules of eosinophils, is cytotoxic to tracheal epithelium. However, the extent of this e€ect on other cell types has not been evaluatedin vitro.Inthis study, we evaluated the e€ect of ECP on 13 mammalian cell lines. ECP inhibited the growth of several cell lines including those derived from carcinoma and leukemia in a dose-dependent manner.

10p research12 29-04-2013 36 4   Download

Annexin A1 (ANX-1), a calcium-dependent, phospholipid binding protein, is known to be involved in diverse cellular processes, including regulation ofcell growth and differen-tiation, apoptosis, and inflammation. The mitogen phorbol 12-myristate 13-acetate (PMA) induces expression and phosphorylation ofANX-1. However, the roles of ANX-1 in PMA-induced signal transduction is unknown. Here, we study the cellular localization ofANX-1 in the PMA-induced signal transduction process.

6p tumor12 20-04-2013 31 4   Download

Functional cooperationbetween integrins andgrowth factor receptors has been reported for several systems, oneofwhich is the modulation of insulin signaling by avb3 integrin. Plasminogen activator inhibitor type-1 (PAI-1), competes withavb3 integrin for vitronectin (VN) binding. Here we report that PAI-1, in a VN-dependent manner, prevents the cooperation of avb3 integrin with insulin signaling in NIH3T3 fibroblasts, resulting in a decrease in insulin-induced protein kinase B (PKB) phosphorylation, vascular endothelial growth factor (VEGF) expression and cell migration. ...

8p tumor12 20-04-2013 35 3   Download

To sustain life mammals have an absolute and continual requirement for oxygen, which is necessary to produce energy for normal cell survival and growth. Hence, main-tainingoxygen homeostasis is a critical requirement and mammals have evolved a wide range of cellular and phy-siological responses to adapt to changes in oxygen avail-ability. In the past few years it has become evident that the transcriptional protein complex hypoxia-inducible factor (HIF) is a key regulator of these processes.

10p tumor12 20-04-2013 35 4   Download

Thyroid hormone has various effects on cell proliferation, growth and apoptosis. To gain more insight into the molecular dynamics caused by thyroid hormone, gene expression in HeLaTRcells that constitutively over-expressed the thyroid hormone receptor (TR) was analyzed. Gene expression profiling of the HeLaTRcells with an oligonucleotide microarray yielded 229 genes whose expression was significantly altered by T3.

10p fptmusic 16-04-2013 36 4   Download

HR12 is anovel farnesyltransferase inhibitor (FTI).Wehave shown previously that HR12 induces phenotypic reversion of H-ras V12 -transformed Rat1 (Rat1/ras) fibroblasts. This reversion was characterized by formation of cell–cell con-tacts, focal adhesions and stress fibers. Here we show that HR12 inhibits anchorage independent and dependent growth of Rat1/ras cells. HR12 also suppresses motility and proliferation of Rat1/ras cells, in a wound healing assay. Rat1 fibroblasts transformed with myristoylated H-ras V12 (Rat1/myr-ras) were resistant to HR12....

14p fptmusic 16-04-2013 38 2   Download

Nitric oxide (NO • ) strongly inhibits the proliferation of human A431 tumour cells. It also inhibits tyrosine phos-phorylation of a 170-kDa band corresponding to the epidermal growth factor receptor (EGFR) and induces the phosphorylation at tyrosine residue(s) of a 58-kDa protein which we have denoted NOIPP-58 (nitric oxide-induced 58-kDa phosphoprotein). The NO • -induced phosphoryla-tion of NOIPP-58 is strictly dependent on the presence of EGF. Phosphorylation of NOIPP-58 and inhibition of the phosphorylation of the band corresponding to EGFR are both cGMP-independent processes. ...

10p fptmusic 16-04-2013 29 3   Download

Gprotein-coupled receptor 30 (GPR30) has previously been described tobe important in steroid-mediatedgrowthand to inhibit cell proliferation. Here we investigated whether the effect of GPR30 on cell growth is dependent on steroid hormone receptors. We stably introduced GPR30 in immortalizednormalmammaryepithelial (HME) cellsusing retroviruses for gene delivery. GPR30 inhibited the growth and proliferation of the cells. They expressed glucocorticoid receptor, but not estrogen or progesterone receptor....

10p awards 05-04-2013 49 6   Download

The mechanism by which the CXC chemokine platelet fac-tor 4 (PF-4) inhibits endothelial cell proliferation is unclear. The heparin-binding domains of PF-4 have been reported to prevent vascular endothelial growth factor 165 (VEGF165 ) andfibroblast growth factor 2 (FGF2) frominteractingwith their receptors. However, other studies have suggested that PF-4 acts via heparin-binding independent interactions. Here, we compared the effects of PF-4 on the signalling events involved in the proliferation induced by VEGF165 , which binds heparin, and by VEGF121 , which does not....

0p awards 05-04-2013 37 4   Download

SUT2was found ina screen formulticopy suppressorsof the synthetic slow growth phenotype of aDras2Dgpa2double deletion mutant. It failed, however, to cure the lethal phenotype of aDras1Dras2mutant suggesting that it acts upstreamof Ras or in a parallel pathway. By testing cAMP-dependent reactions including the accumulation of storage carbohydrates, pseudohyphal differentiation, entry of mei-osis as well as the measurement ofFLO11reporter activity we show that Sut2pmodulates the activity of protein kinase A (PKA)....

8p dell39 03-04-2013 39 3   Download