Development of a lateral flow immunoassay test strip

Among several methods to detect the toxic and cancerous aflatoxins in agricultural products used in foods and animal feeds, only the lateral flow immunodipstick method is suitable for field usage. The aim of this study is to determine optimal conditions for the conjugation of aflatoxin B1 (AFB1) with bovine serum albumin (BSA) to develop a lateral flow immunoassay test strip for detection of AFB1.

9p viuzumaki2711 08-05-2019 13 0   Download

This study was designed to construct a lateral flow immunoassay test strip for rapid detection of rubella virus. Rabbit anti-rubella antibody and pig anti-rubella antibody were successfully purified from sera of rabbit and pig infected with rubella virus. The yields of IgG affinity purification were 1.74 mg/ml and 3.23 mg/ml serum, respectively.

6p trangcham1896 10-12-2018 40 1   Download

In the study, we showed the result of covalent conjugation of antirotavirus antibody and AuNP for generating a lateral flow immunoassay strip to detect rotavirus in fecal samples. The suitable conditions for coating polyethylene glycol (PEG) on the surface of AuNP were 10.0 M PEG for 3 hours at room temperature (25 oC). Optimized conditions for covalent conjugation of antibody and AuNP were pH 4.0, 0.1 g antibody/conjugate, 0.01 mM reactant EDC/NHS [1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride/(N-hydroxy sulfosuccinimide].

8p cumeo3000 01-08-2018 40 0   Download