Development of haploid

Plant height is a prime example of a dynamic trait that changes constantly throughout adult development. In this study we utilised a large triticale mapping population, comprising 647 doubled haploid lines derived from 4 families, to phenotype for plant height by a precision phenotyping platform at multiple time points.

8p vinarcissa 21-03-2023 9 1   Download

Farmed Atlantic salmon are one of the most economically significant global aquaculture products. Early sexual maturation of farmed males represents a significant challenge to this industry and has been linked with the vgll3 genotype.

13p vihagrid 30-01-2023 8 3   Download

Generating chromosome-level, haplotype-resolved assemblies of heterozygous genomes remains challenging. To address this, we developed gamete binning, a method based on single-cell sequencing of haploid gametes enabling separation of the whole-genome sequencing reads into haplotype-specific reads sets.

20p viarchimedes 26-01-2022 10 0   Download

De novo mutations (DNMs) are associated with neurodevelopmental and congenital diseases, and their detection can contribute to understanding disease pathogenicity. However, accurate detection is challenging because of their small number relative to the genome-wide false positives in next generation sequencing (NGS) data. Software such as DeNovoGear and TrioDeNovo have been developed to detect DNMs, but at good sensitivity they still produce many false positive calls.

7p vibeauty 23-10-2021 8 1   Download

Nutrition and cell size play an important role in the determination of caste differentiation in queen and worker of honeybees (Apis mellifera), whereas the haploid genome dominates the differentiation of drones. However, the effects of female developmental environment on the development of males remain unclear. In this study, young drone larvae were transferred into worker cells (WCs) or remained in drone cells (DCs) to rear drones.

12p vitzuyu2711 29-09-2021 7 1   Download

Two-line hybrid wheat system using thermo-photo sensitive genic male sterility (TPSGMS) is currently the most promising approach for wheat heterosis utilization in China. However, during past 20 years only few TPSGMS lines were developed in hybrid wheat breeding, which has been the main limiting factor to create heterotic hybrids.

10p vijichea2711 28-05-2021 10 1   Download

The aim of this study was to develop a culture medium for the production of double haploids from local chilli varieties in Vietnam which provides an opportunity to shorten the breeding cycle and fix agronomic traits.

8p nguaconbaynhay11 16-04-2021 11 2   Download

Rapeseed (Brassica napus L.) is an important oilseed crop throughout the world, serving as source for edible oil and renewable energy. Development of nested association mapping (NAM) population and methods is of importance for quantitative trait locus (QTL) mapping in rapeseed.

17p vihashirama2711 21-05-2020 10 1   Download

Microspore embryogenesis describes a stress-induced reprogramming of immature male plant gametophytes to develop into embryo-like structures, which can be regenerated into doubled haploid plants after whole genome reduplication. This mechanism is of high interest for both research as well as plant breeding.

16p vihashirama2711 21-05-2020 14 1   Download

Anther Culture or microspore culture is a very important and useful tool in plant breeding for efficient production of haploids and subsequent doubled haploid plants in many species, limited research has been conducted with Brassica –rapaas it is more recalcitrant in cell and tissue culture than other Brassicas. In order to access the effect of genotype on microspore embryogenesis in B –rapa, two high yielding brown Sarsoon cultivars (KS-101 and KOS-1) were studied for their response to haploid plant regeneration through Anther culture. Flower buds of 2.2 mm to 2.

4p nguaconbaynhay5 11-05-2020 9 0   Download

Homozygosity at a particular locus is always desirable feature for crop improvement programs. Breeding is one of the approaches to attain this but it requires minimum 6-7 cropping years. Due to this limitation it is only feasible in annuals. As most of the fruit crops are not annuals, they grow in vegetative phase for 5-6 year before flowering to come. Hence, to achieve homozygosity through breeding approaches is not feasible in them. Use of haploid to produce double haploid is one of the potent approaches to overcome this lacuna.

14p angicungduoc4 26-04-2020 12 1   Download

Marigold is an important ornamental plant grown as garden plant and for flower decoration. It has been recognized as a key source of carotenoids; exclusively used in Pharmaceutical industries and poultry feed additives. However, improvement of this valuable species is hampered by its high heterozygosity, but presently the greatest emphasis is on development of haploids through ovary culture, the efficient and timesaving technique in varietal improvement.

11p quenchua4 06-04-2020 23 2   Download

A doubled haploid (DH) is a genotype developed when haploid cells undergo chromosome doubling. Artificial production of doubled haploids is important in plant breeding. Double haploid (DH) breeding can helps in speedup conventional plant breeding programmes and make early release of cultivars with superior and desirable traits along with greater utility in other research aspects of plant breeding, genetics and genetic engineering. DH’s helps in complementing back cross breeding by transferring genes of interest from wild relatives thus breaking genetic barriers.

18p nguaconbaynhay3 07-02-2020 20 0   Download

Fourteen genotypes and 3 different gamma ray doses were tested to develop an efficient haploidization protocol in squash. For this purpose, male flowers collected 1 day before anthesis were irradiated with 150, 200, and 300 Gy gamma ray doses, and female flowers were pollinated with the irradiated pollens the next day.

10p sony2711 01-02-2019 26 2   Download

Doubled haploid (DH) plant production plays an important role in pepper (Capsicum annuum L.) breeding for development of new varieties. However, information is lacking for DH plant production in pepper genotypes carrying ornamental value. The major goal of this study was to produce DH ornamental pepper lines having the mentioned value.

11p sony2711 28-01-2019 25 1   Download

This is the first report of successful regeneration of haploid lines in iris (Iris pseudacorus) developed by in situ parthenogenesis followed by embryo rescue. Native genotypes of I. pseudacorus (2n = 38) were prepared as female parents and I. spuria was set as the males.

5p danhdanh11 11-01-2019 23 2   Download

The genomics revolution of the past decade has greatly enhanced our understanding of the genetic composition of living organisms including many plant species of economic importance. Complete genomic sequences of Arabidopsis and several major crops, together with high-throughput technologies for analyses of transcripts, proteins and mutants, provide the basis for understanding the relationship between genes, proteins and phenotypes.

755p diennmri 19-09-2013 94 22   Download

Cells of the budding yeast, S. cerevisiae, have for several decades now been considered as the prototypic eukaryotic cells, ideally suited to study and uncover many of the basic phenomena of eukaryotic life.

378p hotmoingay 04-01-2013 49 4   Download

Genetic information is physically carried on large DNA strings that are organized into chromosomes. Each species is characterized by a chromosome set that carry the information necessary and sufficient for its development and survival. Eukaryotic organisms are mostly diploid, containing two sets of chromosomes with each pair carrying nearly identical genetic information. Occasionally, exceptions to this rule are found, such as haploid yeast (with only one set of chromosomes) or polyploid ferns and frogs (with multiple sets).

256p wqwqwqwqwq 09-07-2012 60 11   Download

The production of ova and sperm occurs via the process of meiosis (whereas somatic cells undergo division via mitosis). Oogenesis produces ova, and spermatogenesis produces sperm. One spermatogonium results in four sperm, and one oogonium results in one ovum and two polar bodies. Meiosis is a reduction division normally allowing each gamete to contain 23 chromosomes (haploid). Thus, when fertilization occurs and the two haploid gametes unite, the resulting zygote contains 46 chromosomes (diploid) under normal circumstances. Two meiotic divisions occur, and each contains several stages. ...

20p lananhanh123 27-08-2011 63 3   Download