Differential splicing

Despite the many approaches to study differential splicing from RNA-seq, many challenges remain unsolved, including computing capacity and sequencing depth requirements. Here we present SUPPA2, a new method that addresses these challenges, and enables streamlined analysis across multiple conditions taking into account biological variability.

11p vimichaelfaraday 25-03-2022 20 1   Download

In humans, thromboxane (TX) A2 signals through two TXA2receptor (TP) isoforms, TPaand TPb,thatdiverge within their carboxyl terminal cytoplasmic (C) tail regions and arise by differential splicing. The human TP gene con-tains three exons E1–E3; while E1 exclusively encodes 5¢ untranslatedregion (UTR) sequence,E2andE3 represent the main coding exons. An additional noncoding exon, E1b was identified within intron 1. Additionally, the TP gene contains twopromoters P1 andP2 located 5¢of E1 andE1b, respectively....

16p research12 23-04-2013 42 3   Download

The intracellular role of placental protein 17b (PP17b)/ TIP47has been controversial, because it is considered tobe a protein required for mannose 6-phosphate receptor trans-port from endosome totrans-Golgi as well as a neutral lipid droplet-associatedprotein.The similaritybetween theamino acid sequences of PP17 variants, adipophilin and perilipins, andbetween their gene structures indicate that PP17baswell as other alternatively spliced PP17 variants belong to the lipid storage droplet protein family, containing also some differentiation factors....

13p tumor12 20-04-2013 38 3   Download

The TPaand TPbisoforms of the human thromboxane A2 receptor (TP) arise by differential splicing but are under the transcriptional control of two distinct promoters, termed Prm1 and Prm3, respectively (Coyle et al. 2002Eur J Biochem269, 4058–4073). The aim of the current study was to determine the key factors regulating TPbexpression by functionally charac-terizing Prm3, identifying the core promoter and the cis-acting elements regulating basal Prm3 activity.

18p awards 05-04-2013 40 3   Download

We used differential scanning calorimetry (DSC) and circular dichroism (CD) to investigate thermal unfolding of recombinant fibroblast isoforms ofa-tropomyosin (Tm) in comparison with that of smooth muscle Tm. These two nonmuscle Tm isoforms 5a and 5b differ internally only by exons 6b⁄6a, and they both differ from smooth muscle Tm by the N-terminal exon 1b which replaces the muscle-specific exons 1a and 2a.

13p dell39 27-03-2013 39 3   Download

It has long been considered that cryptic splice sites are ignored by the splic-ing machinery in the context of intact genuine splice sites. In the present study, it is shown that cryptic splice sites are utilized in all circumstances, when the authentic site is intact, partially functional or completely abol-ished.

13p media19 06-03-2013 30 2   Download

Signal transducers and activators of transcription (STATs) regulate a vari-ety of cellular functions, including differentiation and proliferation. STAT3 and STAT5 are known to play important roles in brain processes, such as energy homeostasis and neuronal development. We isolated a novel splicing variant ofSTAT5Afrom a cDNA library of the mouse brainstem.

12p viettel02 20-02-2013 32 4   Download

Unusually among ATP-binding cassette proteins, the sulfonylurea receptor (SUR) acts as a channel regulator. ATP-sensitive potassium channels are octameric complexes composed of four pore-forming Kir6.2 subunits and four regulatory SUR subunits. Two different genes encode SUR1 (ABCC8) and SUR2 (ABCC9), with the latter being differentially spliced to give SUR2A and SUR2B, which differ only in their C-terminal 42 amino acids.

9p mobifone23 21-01-2013 53 6   Download

ProteinN-arginine methyltransferases (PRMTs) participate in a number of cellular processes, including cell growth, nuclear⁄cytoplasmic protein shut-tling, differentiation, RNA splicing and post-transcriptional regulation. PRMT2 (also known as HRMT1L1) is clearly involved in lung function, the inflammatory response

20p cosis54 08-12-2012 38 2   Download

Tham khảo luận văn - đề án 'báo cáo y học: " differential splicing of the apoptosis-associated speck like protein containing a caspase recruitment domain (asc) regulates inflammasomes"', luận văn - báo cáo phục vụ nhu cầu học tập, nghiên cứu và làm việc hiệu quả

13p thulanh27 12-12-2011 72 4   Download

Tuyển tập báo cáo các nghiên cứu khoa học quốc tế ngành y học dành cho các bạn tham khảo đề tài: Differential splicing of the apoptosis-associated speck like protein containing a caspase recruitment domain (ASC) regulates inflammasomes...

13p thulanh26 09-12-2011 47 5   Download

Tuyển tập các báo cáo nghiên cứu về y học được đăng trên tạp chí y học Minireview cung cấp cho các bạn kiến thức về ngành y đề tài: Probe-level estimation improves the detection of differential splicing in Affymetrix exon array studies...

0p thulanh21 12-11-2011 42 3   Download

VEGF and its receptors are required for vasculogenesis (the de novo formation of blood vessels from differentiating endothelial cells, as occurs during embryonic development) and angiogenesis under normal (wound healing, corpus luteum formation) and pathologic processes (tumor angiogenesis, inflammatory conditions such as rheumatoid arthritis).

5p konheokonmummim 03-12-2010 76 6   Download