Dissociation constants

The structure of the new acid and data of potentiometric titration were analyzed in detail before calculating and assigning the pKa values to suitable functional groups. These results will be applied to our further studies of this compound.

7p vimaryamnawaz 04-08-2022 9 1   Download

The effect of some organic compounds on the activity of chitinase from Serratia marcescens has been investigated. Among these compounds, 1,10-phenanthroline was found to be an inhibitor and ethanol - an activator of the chitinase activity;
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’ dipyridyl and Zn2+ do not take any effect on the chitinase activity. In this investigation, the dissociation constant of an chitinase-metalic ion complex (K1) and the inhibition constant (K2) have been estimated with K1 = 0.1 mcM and K2 = 2.667 mM.

5p uocvong04 24-09-2015 39 4   Download

Visual arrestin is converted from a ÔbasalÕ state to an ÔactivatedÕ state by interaction with the phosphorylated C-terminus of photoactivated rhodopsin (R*), but the conformational changes in arrestin that lead to activation are unknown. Small-angle X-ray scattering (SAXS) was used to investigate the solution structure of arrestin and characterize changes attendant upon activation. Wild-type arrestin forms dimers with a dissociation constant of 60 lM.

9p system191 01-06-2013 53 3   Download

Duckd2-crystallin is a soluble tetrameric lens protein. In the presence of guanidinium hydrochloride (GdnHCl),it undergoes stepwise dissociation and unfolding. Gel-filtra-tion chromatography and sedimentation velocity analysis has demonstrated the dissociation of the tetramer protein to a monomeric intermediate with a dissociation constant of 0.34lM 3 . Dimers were also detected during the dissociation and refoldingprocesses.

8p tumor12 20-04-2013 33 1   Download

In the search for new ligands for the plasminogen kringle 4 binding-protein tetranectin, it has been found by ligand blot analysis andELISAthat tetranectinspecificallybound to the plasminogen-like hepatocyte growth factor and tissue-type plasminogen activator.The dissociation constants of these complexes were found to be within the same order of mag-nitude as the one for the plasminogen-tetranectin complex. The study also revealed that tetranectin did not interact with the kindred proteins: macrophage-stimulating protein, urokinase-type plasminogen activator and prothrombin....

5p fptmusic 16-04-2013 51 4   Download

The phosphoproteins HPrSerP and CrhP are the main effectors for CcpA-mediated carbon catabolite regulation (CCR) inBacillus subtilis. Complexes of CcpA with HPrSerP or CrhP regulate genes by binding to the catabolite responsive elements (cre). We present a quantitative analysis of HPrSerP and CrhP interaction with CcpA by surface plasmon resonance (SPR) revealing small and similar equilibrium constants of 4.8 ± 0.4 lm for HPrSerP–CcpA and 19.1 ± 2.5 lmfor CrhP–CcpA complex dissoci-ation.

12p awards 06-04-2013 39 2   Download

The ability ofEntamoeba histolyticaTATA binding protein (EhTBP) to interact with different TATA boxes in gene promoters may be one of the key factors to perform an efficient transcription in this human parasite. In this paper we used several TATA variants to study the in vitro EhTBP DNA-binding activity and to determine the TATA-EhTBP dissociation constants. The presence of EhTBP in complexes formed by nuclear extracts (NE) and the TATTTAAA oligonucleotide, which corresponds to the canonical TATA box for E. histolytica, was demonstrated by gel-shift assays. ...

13p awards 06-04-2013 45 1   Download

The different effects of pH and NaCl on individual O2-binding properties ofaandbsubunits within liganded tetramer and dimer of human hemo-globin (HbA) were examined in a number of laser time-resolved spectro-scopic measurements. A previously proposed approach [Dzhagarov BM & Lepeshkevich SV (2004)Chem Phys Lett390, 59–64] was used to determine the extent of subunit dissociation rate constant difference and subunit affinity difference from a single flash photolysis experiment.

11p dell39 27-03-2013 48 4   Download

Dissociation Constants Of Organic Acids And Bases: This table lists the acid-base dissociation constants of over 600 organic compounds, including many amino acids. All data apply to dilute aqueous solutions and are presented in the form of pKa, which is the negative of the logarithm of the acid dissociation constant Ka.

17p anhngoc007 23-11-2010 363 46   Download