DNA isolation and PCR

Part 1 book "Protocols for the diagnosis of pig viral diseases" includes content: Requirements and preparedness for attending a viral disease outbreak in pig farms; collection of samples, their preservation and transportation; methods for quantification of viruses; protocols for isolation of genetic materials from RNA viruses; multiplex pcr for diagnosis of porcine diseases; protocols for isolation of plasmid DNA,... and other contents.

203p muasambanhan02 18-12-2023 6 3   Download

Part 1 book "Environmental microbiology - Methods and protocols" includes content: Methods for isolation and cultivation of filamentous fungi, rapid extraction of PCR-competent DNA from recalcitrant environmental samples, quantitative PCR for detection of mRNA and gDNA in environmental isolates, analysis of community dynamics in environmental samples using denaturing gradient gel electrophoresis, terminal restriction fragment length polymorphism (T-RFLP) profiling of bacterial 16S rRNA genes,... and other contents.

134p oursky10 04-12-2023 4 2   Download

Part 1 book "PCR Protocols" includes content: Long range PCR with a DNA polymerase fusion, isolation of genomic insertion sites of proviruses using splinkerette PCR based procedures, lariat dependent nested PCR for flanking sequence determination, a global single-Cell cDNA amplification method for quantitative microarray analysis,...and other contents.

171p oursky06 17-10-2023 5 1   Download

(BQ) Ebook Plant Reverse Genetics: Methods and Protocols – Part 2 presents the following content: Chapter 10: methods for rice phenomics studies; chapter 11 : development of an efficient inverse pcr method for isolating gene tags from t-dna insertional mutants in rice; chapter 12: transposon insertional mutagenesis in rice; chapter 13: reverse genetics in medicago truncatula using tnt1 insertion mutants; chapter 14: screening arabidopsis genotypes for drought stress resistance; chapter 15 : protein tagging for chromatin immunoprecipitation from arabidopsis.

151p runordie7 30-08-2022 5 2   Download

The results showed that the designed primers were specific to only Foc isolates from race 1 and tropical race 4 (TR4). The detection efficiency of the designed primers was compared to other published primers through optimized SYBR green-based real-time PCR assay and nested PCR. The new primers could detect the Foc genomic DNA at a minimum of 5 pg and target pathogen in a symptomless banana sucker. The specificity and sensitivity of the new primers were comparable to the published real-time PCR primers and the nested PCR assay.

5p mudbound 10-12-2021 17 1   Download

Soybean homeobox gene GmSBH1 has previously been proven to be involved in response to high temperature and humidity (HTH) stress. To investigate its expression patterns and active cis-elements, a 2040-bp 5’-upstream genomic DNA fragment of GmSBH1, named GmSBH1P, was isolated by PCR walking and characterized. Sequence analysis revealed that the fragment contains a series of cis-acting elements related to stress responses.

13p tudichquannguyet 29-11-2021 9 1   Download

Development and application of DNA-based methods to distinguish highly virulent isolates of Fusarium oxysporum f. sp. koae [Fo koae; cause of koa wilt disease on Acacia koa (koa)] will help disease management through early detection, enhanced monitoring, and improved disease resistance-breeding programs.

15p vijeeni2711 30-06-2021 9 1   Download

This study is planned to provide data on disease prevalence using Nested-PCR amplification based on Internal Transcribed Spacer 1 (ITS-1) and TeRoTat primers, and to investigate genetic characterization and phylogenesis of T. evansi isolates from naturally infected camels in Jazan region, south western Saudi Arabia.

12p trinhthamhodang11 27-04-2021 11 2   Download

Genomic DNA was isolated by CTAB method and then subjected to PCR amplification by using 22 SSR primers. Among 22 primers, 17 primers were amplified. The PIC values were ranged from 0.46 (Phi046) which is the minimum value to 0.81(Phi059) which is the highest value. The frequencies of alleles were ranged from 0.01 to 0.07.

7p nguaconbaynhay7 15-08-2020 16 1   Download

Salmonellosis is one of the most common causal food borne disease in India. An in-house loop-mediated isothermal amplification (LAMP) reaction was established and evaluated for sensitivity and specificity in detecting the presence of Salmonella spp. isolates in foods of animal origin from Mumbai, India. The aim of this study was to develop in-house LAMP for simple and inexpensive detection of Salmonella spp. in animal origin foods using specifically designed primers targeting invA gene contains sequences unique to this genus. The reaction was optimized using genomic DNA of S.

10p angicungduoc6 20-07-2020 23 1   Download

Molecular characterization of the Salmonella spp isolated from small ruminants in and around Bidar. A total of 140 fecal samples collected were from sheep and goats. Based on the morphological, cultural and biochemical characterization indicated that 8 isolates were Salomnella spp. Biochemically confirmed isolates were subjected to invA gene based PCR and diagnostic amplicon specific to virulence gene of 284 bp was evident in all the 8 Salmonella isolates indicating potential carriage of invasion properties linked to virulence.

6p kethamoi6 29-06-2020 13 2   Download

A simple, rapid, safe and cost effective DNA extraction method is an all time need for efficient molecular screening of fungus using polymerase chain reaction (PCR). A number of protocols are now available to suit recovery of DNA from different fungi. But, only a few are universally used for all fungal origin. In this pursuit, the authors presented a detailed review of the status of fungal DNA isolation using different methods.

10p chauchaungayxua6 26-06-2020 24 1   Download

Totally 32 weed hosts and crop plants were collected and the DNA was isolated from 32 weeds and crops showing phytoplasma disease symptoms and subjected to PCR amplification with phytoplasma specific primers R16F2n/R16R2. The result shows that R16F2n/R16R2 primer amplified 1250bp product in 19 weeds and other crop species. The notable contribution in the present study was identification of six new hosts for phytoplasma for the first time in the Andhra Pradesh.

11p angicungduoc5 14-06-2020 8 1   Download

Twenty isolates of Sclerotium rolfsii Sacc. collected from different hosts and locations of India was studied in relation to genomic DNA amplification through internal transcribed spacer (ITS-PCR) analysis. These isolates of S. rolfsii showed variation at rDNA level which was revealed through ITS1-5.8s-ITS 4 primer series.

10p trinhthamhodang1213 30-05-2020 8 0   Download

The present study was carried out for the isolation, identification and molecular characterization of Brucella species. A total of 50 samples were collected from cattle and buffalo suffering from abortions and other reproductive disorders in and around Ludhiana, Punjab. Out of the 50 samples of fetal stomach contents (25), uterine discharges (10), vaginal mucus (8) and placenta (7) processed for isolation of Brucella of which a total of four isolate were obtained and identified biochemically. All the 4 isolates were typed as biotype 1.

5p trinhthamhodang1213 29-05-2020 6 0   Download

The robust technique of polymerase chain reaction (PCR) has revolutionized the field of plant molecular biology and has made the molecular characterization of crop plants easy, rapid and reproducible. Only a minute amount of input genomic DNA is required in PCR. But, the isolated DNA must be free from different contaminants, which can potentially act as PCR inhibitors. The efficiency of PCR might be reduced in case of the medicinal and aromatic plants, due to the presence of biomolecules acting as PCR inhibitors.

5p cothumenhmong5 17-05-2020 16 0   Download

The effectiveness of genotyping for any genetic studies relies on the quantity and quality of DNA isolated. The DNA isolation procedures differs for different crop species depending upon the phytochemical composition of the tissue used for isolation of DNA. The polyphenol abundance in Lesser yam interferes in the isolation of high quality DNA. The quantitative and qualitative assessment of DNA isolated using different extraction methods therefore becomes a priority. The selection of accurate isolation method becomes absolutely essential to obtain PCR amplification.

11p trinhthamhodang5 16-05-2020 7 0   Download

In this study, RAPD fingerprinting has been carried out for variants selected from orchards growing with micropropagated Robusta banana (Musa spp. ‘AAA’) plants. After isolation of quality DNA and preliminary screening of random primers, fourteen arbitrary (10-mers) primers showing intense unambiguous and reproducible PCR amplification have been selected for study. Among them OPA-19 and OPC-03 showed only monomorphic bands while OPA-02, OPA-09, OPA-13, OPA-14, OPB-6, OPB-15, OPC-01, OPD-10, OPF-04 and OPF-12 primers showed polymorphic bands.

10p nguaconbaynhay5 16-05-2020 11 0   Download

Six isolates of chickpea collar rot fungus of Sclerotium rolfsii were collected from different locations of Maharashtra were investigated for genetic diversity under present experiment. We employed five SSR of MB- series to construct a genotype-specific DNA fragment profile of field isolates of this fungus. The PCR amplified product of each primer was resolved on 10 % Polyacrylamide gel electrophoresis. The 5 SSR primers screened produced a total of 60 reproducible and scorable amplicons. The size of amplicons produced ranged from 115bp to 800bp.

8p chauchaungayxua5 05-05-2020 17 2   Download

The present investigation entitled “Optimization Protocol of DNA isolation and PCR in Muskmelon (Cucumis melo L.) by RAPD Marker” was carried out at Department of Plant Biotechnology SDMVM’s College of Agricultural Biotechnology, Georai Tanda, Paithan Road, Aurangabad (M.S.), 431001. Six different commercial verities of muskmelon plant were studied for this research.

7p kequaidan4 05-05-2020 7 1   Download