Doubled haploid production

Ebook "Progress and opportunities of doubled haploid production" deals with the historical perspectives and the current status of doubled haploid production along with its practical implications in basic and applied research. It highlights various haploid production methods with a comprehensive discussion on their pros and cons, bottlenecks, and embryogenic pathways.

84p manmanthanhla0201 26-02-2024 4 1   Download

In several fish species homozygous and heterozygous clonal lines have been produced using gynogenetic and androgenetic techniques. These lines are standardized and can be reproduced over generations. In rainbow trout such lines have existed for decades and has become important research tools in genome studies as well as in studies of commercially important traits.

10p vihagrid 30-01-2023 7 3   Download

Farmed Atlantic salmon are one of the most economically significant global aquaculture products. Early sexual maturation of farmed males represents a significant challenge to this industry and has been linked with the vgll3 genotype.

13p vihagrid 30-01-2023 8 3   Download

Anther culture response of six wheat genotypes and their F1 crosses was investigated. Results indicated that considerable genetic variation among the tested genotypes was observed. Calli were obtained from all wheat genotypes studied.

10p schindler 30-12-2021 5 0   Download

Low temperatures decrease the capacity for biomass production and lead to growth retardation up to irreversible cellular damage in modern maize cultivars. European flint landraces are an untapped genetic resource for genes and alleles conferring cold tolerance which they acquired during their adaptation to the agroecological conditions in Europe.

15p vijeeni2711 24-07-2021 8 0   Download

Artificial synthesis of octoploid rapeseed double haploid (DH) induction lines Y3380 and Y3560 was made possible by interspecific hybridization and genome doubling techniques. Production of pure lines by DH induction provides a new way to achieve homozygosity earlier in B.napus.

13p vijichea2711 28-05-2021 19 1   Download

Microspore embryogenesis is potentially the most effective method of obtaining doubled haploids (DH) which are utilized in breeding programs to accelerate production of new cultivars.

24p vijichea2711 28-05-2021 5 1   Download

The aim of this study was to develop a culture medium for the production of double haploids from local chilli varieties in Vietnam which provides an opportunity to shorten the breeding cycle and fix agronomic traits.

8p nguaconbaynhay11 16-04-2021 11 2   Download

Five different emasculation methods viz. standard technique for wheat x maize crosses, early pollinations on non-emasculated spikes, clipped glume facilitated emasculation, plastic bag emasculation and hot water emasculation were compared. Clipped glume facilitated emasculation resulted in lowest caryopses formation frequency. Early pollination on non-emasculated spikes gave reasonably high embryo formation frequency while omitting tedious emasculation steps.

8p nguaconbaynhay7 15-08-2020 15 1   Download

Sporophytic plants having gametophytic chromosome number are termed as haploid. These plants can be produced either spontaneously in nature or by in vitro or in vivo induction technique. By doubling chromosome number of haploid either spontaneously or artificially, double haploid (DH) could be produced. Double haploid are homozygous and this homozygosity is achieved in one generation by using DH production systems. This production system is used for fixation of heterosis. Genetic map construction is relatively easy with DH population.

15p nguaconbaynhay5 16-05-2020 7 1   Download

Anther Culture or microspore culture is a very important and useful tool in plant breeding for efficient production of haploids and subsequent doubled haploid plants in many species, limited research has been conducted with Brassica –rapaas it is more recalcitrant in cell and tissue culture than other Brassicas. In order to access the effect of genotype on microspore embryogenesis in B –rapa, two high yielding brown Sarsoon cultivars (KS-101 and KOS-1) were studied for their response to haploid plant regeneration through Anther culture. Flower buds of 2.2 mm to 2.

4p nguaconbaynhay5 11-05-2020 9 0   Download

Homozygosity at a particular locus is always desirable feature for crop improvement programs. Breeding is one of the approaches to attain this but it requires minimum 6-7 cropping years. Due to this limitation it is only feasible in annuals. As most of the fruit crops are not annuals, they grow in vegetative phase for 5-6 year before flowering to come. Hence, to achieve homozygosity through breeding approaches is not feasible in them. Use of haploid to produce double haploid is one of the potent approaches to overcome this lacuna.

14p angicungduoc4 26-04-2020 12 1   Download

Conventional breeding involves time taking procedure for pyramiding of genes and screening of crops for the same. Double haploid (DH) production can reduce the time of production of resistant crops in less time period. Twenty one plants that survived after the hardening procedure were screened phenotypically and molecularly for BB resistant genes.

8p nguaconbaynhay3 07-02-2020 14 0   Download

A doubled haploid (DH) is a genotype developed when haploid cells undergo chromosome doubling. Artificial production of doubled haploids is important in plant breeding. Double haploid (DH) breeding can helps in speedup conventional plant breeding programmes and make early release of cultivars with superior and desirable traits along with greater utility in other research aspects of plant breeding, genetics and genetic engineering. DH’s helps in complementing back cross breeding by transferring genes of interest from wild relatives thus breaking genetic barriers.

18p nguaconbaynhay3 07-02-2020 20 0   Download

An anther culture method was used for the production of doubled haploids (DHs) in Lithuanian barley and potato cultivars that were directly regenerated from embryoids (Caredda’s method) were applied to determine androgenic potential according to the green regenerant yield and other morphogenetic factors.

6p vitamix 15-02-2019 14 0   Download

Doubled haploid (DH) plant production plays an important role in pepper (Capsicum annuum L.) breeding for development of new varieties. However, information is lacking for DH plant production in pepper genotypes carrying ornamental value. The major goal of this study was to produce DH ornamental pepper lines having the mentioned value.

11p sony2711 28-01-2019 25 1   Download

To create new varieties resistant to biotic and abiotic stress factors that possess high productivity and improved fruit quality, breeders require modern breeding methods and techniques. Double haploid (DH) lines are an invaluable breeding element due to their guaranteed, completely homozygous nature.

8p sony2711 28-01-2019 17 3   Download

Breeding based on doubled-haploid approaches has recently become a common tool for accelerating crop improvement in many plant species. However, many plant species do not have a reliable method for haploid induction. A promising new approach involving centromere engineering has recently been proposed to overcome this limitation.

6p danhdanh11 11-01-2019 17 1   Download